| In situ observation of C60（C（COOH）2）2 interacting with living cells using fluorescence microscopy |
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| 作者姓名： | YE Chang CHEN Chunying CHEN Zhen MENG Huan XING Li JIANG Yaxin YUAN Hui XING Gengmei ZHAO Feng ZHAO Yuliang CHAI Zhifang FANG Xiaohong HAN Dong CHEN Long WANG Chen WEI Taotao |
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| 作者单位： | [1]Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials, Institute of High Energy of Physics, Chinese Academy of Sciences, Beijing 100049, China [2]Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China [3]National Center for Nanoscience and Technology, Beijing 100080, China [4]Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
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| 基金项目： | Acknowledgements The authors thank Prof. Liu Yuanfang of Peking University for his valuable suggestions and discussions. This work was supported by the National Natural Science Foundation of China （Grant Nos. 10490180 and 90406024）, the 0utstanding Young Natural Scientist Foundation （Grant Nos. 10525524 and 20225516）, the Ministry of Science and Technology （Grant No. 2005CB724703）, and the Directionality Program of the Chinese Academy of Sciences （Grant No. KJCX-SW-H12）. |
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| 摘 要： | 1 Introduction In recent years, studies on biological effects of thenanoscale materials have become the cornerstone of rapidly developed nanomedical and nanobiological technologies. Moreover, studies on the bio-effects when the different kinds of nanoscal…
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| 关 键 词： | C60（C（COOH）2）2 原位观察 真实时间成象 球壳状碳分子 细胞膜 荧光标记 |
| 文章编号： | 10.0007/s11434-006-1060-1 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005-08-222005-11-28 |
In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy |
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| YE Chang CHEN Chunying CHEN Zhen MENG Huan XING Li JIANG Yaxin YUAN Hui XING Gengmei ZHAO Feng ZHAO Yuliang CHAI Zhifang FANG Xiaohong HAN Dong CHEN Long WANG Chen WEI Taotao.In situ observation of C60(C(COOH)2)2 interacting with living cells using fluorescence microscopy[J].Chinese Science Bulletin,2006,51(9):1060-1064. |
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| Authors: | Chang Ye Chunying Chen Zhen Chen Huan Meng Li Xing Yaxin Jiang Hui Yuan Gengmei Xing Feng Zhao Yuliang Zhao Zhifang Chai Xiaohong Fang Dong Han Long Chen Chen Wang Taotao Wei |
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| Institution: | (1) Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials, Institute of High Energy of Physics, Chinese Academy of Sciences, Beijing, 100049, China;(2) Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, China;(3) National Center for Nanoscience and Technology, Beijing, 100080, China;(4) Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China |
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| Abstract: | The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy.
For this purpose, the C60 derivative C60(C(COOH)2)2, a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its
interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results
indicate that C60(C(COOH)2)2 can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C60(C(COOH)2)2 can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter
the cell or adhere to membrane. At concentrations ranging from 1 × 10−2 to 1 × 102 mg/L, C60(C(COOH)2)2 does not show any detectable cytotoxicity. |
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| Keywords: | fluorescence microscopy real time imaging fullerene derivative C60(C(COOH)2)2 cross cellular membrane fluorescence labeling |
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