| 东南景天DNA提取方法的比较 |
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| 引用本文： | 刘顺枝,刘雯,江月玲,郑仙桃,王小兰,胡位荣. 东南景天DNA提取方法的比较[J]. 广州大学学报(综合版), 2012, 0(1): 27-31 |
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| 作者姓名： | 刘顺枝 刘雯 江月玲 郑仙桃 王小兰 胡位荣 |
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| 作者单位： | [1]广州大学生命科学学院,广东广州510006 [2]仲恺农业工程学院环境科学与工程学院,广东广州510225 |
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| 摘 要： | 以超积累生态型、非超积累生态型东南景天（SedumalfrediiHance）的嫩叶为材料,采用CTAB法、SDS法、尿素法、柠檬酸钠法提取DNA,比较不同方法提取DNA的效率．结果表明,采用4种方法提取超积累生态型东南景天和非超积累生态型东南景天的DNA,其OD260／OD280的比值在1．8～2．0之间;除柠檬酸钠法外,其余3种方法提取的DNAOD26。／OD230的比值均大于2．0;其DNA浓度和得率都是尿素法〉CTAB法〉SDS法〉柠檬酸钠法,从琼脂糖凝胶电泳图来看,也是尿素法效果最好．因此,认为尿素法适合于超积累生态型和非超积累生态型东南景天DNA的提取．
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| 关 键 词： | 东南景天 DNA提取方法 浓度 得率 |
Comparison on four extraction methods of genomic DNA from two ecotypes of Sedum alfredii |
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| Affiliation: | LIU Shun-zhi, LIU Wen , JIANG Yue-ling , ZHENG Xian-tao1 , WANG Xiao-lan Wei-rong ( School of Life Sciences, Guangzhou University, Guangzhou 510006, China ; School of Environmental Science and Engineering Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China) |
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| Abstract: | Comparing the efficiency of four DNA extraction methods, i.e. CTAB, SDS, Carbamide and Sodium citrate from Sedum alfredii, the fresh tender leaves of hyperaccumulating and non-hyperaccumulating ecotypes of S. alfredii were used as materials. The results showed that the OD26o/OD2s0 ratio of genomic DNA from both e- cotypes of S. alfredii were ranged between 1.8 and 2.0. Except Sodium citrate, the other 3 extraction methods received the ratio of OD260/OD230 of the genomic DNA larger than 2.0. The DNA concentration and yield ranked as follows： Carbamide 〉 CTAB 〉 SDS 〉 Sodium citrate. The DNA electrophoresis confirmed also that the Car- bamide method was the most efficient DNA extraction for the two ecotypes of S. alfredii. |
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| Keywords: | Sedum alfredii DNA extraction method concentration yield |
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