| 地高辛标记DNA探针应用于凝胶转移电泳 |
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| 引用本文： | 刘冰菁 姜先荣. 地高辛标记DNA探针应用于凝胶转移电泳[J]. 阜阳师范学院学报(自然科学版), 1998, 0(4): 24-29 |
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| 作者姓名： | 刘冰菁 姜先荣 |
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| 作者单位： | [1]阜阳电视大学 [2]安徽教育学院生物系 |
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| 摘 要： | 半抗原DIG-ddUTP与DNA连接,以此作为探针可以与其相识别的蛋白质相结合,再用带有碱性磷酸的抗体处理,形成的复合物可作用于底物CSPD,使其产生萤光,而被x光片记录下来。
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| 关 键 词： | 地高辛标记 DNA探针 凝胶转移电泳 |
The Digoxigenin Labeled DNA Probe Is Applied to Gel Shift Electrophoresis |
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| Liu Bingqing. The Digoxigenin Labeled DNA Probe Is Applied to Gel Shift Electrophoresis[J]. Journal of Fuyang Teachers College:Natural Science, 1998, 0(4): 24-29 |
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| Authors: | Liu Bingqing |
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| Abstract: | Hapten DIG- ddUTP is combined to DNA by terminal transferase. The DNA probe associates with its specific protein. The DNA-Protein is treated with anti-DIG antibody, Fab fragments coupled to alkaline phosphatase, and thereby forms a complex. The complex can catalyze its substrate CSPD to produce chemilu-minescent signals which are recorded by x-ray film. |
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| Keywords: | DIG- label DNA probe Gel shift electrophoresis |
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