| 用CTAB法提取植物DNA的技术改进 |
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| 引用本文： | 陈向明.用CTAB法提取植物DNA的技术改进[J].合肥学院学报(自然科学版),2000(4). |
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| 作者姓名： | 陈向明 |
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| 作者单位： | 合肥教育学院生化系 合肥 |
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| 摘 要： | 本文以富含酚类物质的牡丹叶片为材料,就 CTAB 法提取植物组织中的 DNA 的方法做了进一步的改进,主要涉及二巯基乙醇的用量、氯仿/异戊醇的用量、以及 DNA 晾干方法的改进等,为后续的分子生物学试验提供了纯净的基因组 DNA。
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| 关 键 词： | DNA 提取 CTAB 法 酚类物质 二巯基乙醇 氯仿/异戊醇 |
The technologyimprovement of extracting DNA from plants by CTAB |
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| CHEN Xiang-ming.The technologyimprovement of extracting DNA from plants by CTAB[J].Journal of Hefei University :Natural Sciences,2000(4). |
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| Authors: | CHEN Xiang-ming |
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| Abstract: | Taking peony leaves full of phenol as experimental materials,the improvement of extracting DNA from plant tissues by CTAB,which involves doses of 2-thiclethylal-cohol chloroform/isoamy- lalcohol and improvement of drying DNA in the air,supplies DNA of purified genes for the folliwing molecular biological exeriements. |
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| Keywords: | DNA extraction CTAB phenol 2-thiolethylal-cohol chloroform/isoamylalcohol |
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