黄精丸对大鼠大脑局灶性脑缺血损伤保护作用研究

目的观察黄精丸对大鼠大脑中动脉缺血再灌注损伤的影响。方法大鼠提前30d灌胃黄精丸(相当生药量2~10g/kg)后,采用大鼠局灶性脑缺血再灌注损伤模型,观察大鼠行为学变化、脑组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、脂质过氧化物丙二醛(MDA)、白细胞介素(IL)-1β及肿瘤坏死因子(TNF)-α水平的变化。结果与缺血再灌注损伤组比较,黄精丸组脑功能受损程度明显改善,脑组织SOD与GSH-Px水平显著升高,MDA、IL-1β、TNF-α水平显著下降且呈剂量依赖关系。结论黄精丸对大鼠大脑缺血再灌注诱导的氧自由基、脂质过氧化、各种炎性因子损伤具有良好的保护作用。

THE PROTECTIVE EFFECTS OF HUANG JING WAN ON CEREBRAL INJURY AFTER FOCAL ISCHEMIC REPERFUSION IN RATS

Objective： To study the protective effect of Huang Jing Wan on cerebral injury after focal ischemic reperfusion in rats. Methods： The rats were treated with Huang Jing Wan-liquid at dose 2-10g/kg by intragastric administration 30 days before the animal model was established by middle cerebral artery occlusion（MCAO）, the changes of the neurological deficits scores, the activities of SOD, GSH-Px and the content of MDA, IL-1β, TNF-α were investigated. Results： Compared with the control, the neurological deficits scores of cerebral occlusion index was significantly improved in Huang Jing Wan treatment, SOD and GSH-Px levels were significantly enhanced, MDA, IL-1β, TNF-α levels significantly decreased and depended on the dosage. Conclusion： Huang Jing Wan has the neuroprotective effect against cerebral ischemia-reperfusion in rats via its antioxidant activity.