| 异小杆线虫离体繁殖技术研究 |
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| 引用本文： | 王丽芳,李春杰,许艳丽.异小杆线虫离体繁殖技术研究[J].农业系统科学与综合研究,2010,26(1):31-35. |
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| 作者姓名： | 王丽芳 李春杰 许艳丽 |
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| 作者单位： | 1. 中国科学院,东北地理与农业生态研究所,黑龙江,哈尔滨,150081;中国科学院,研究生院,北京,100049
2. 中国科学院,东北地理与农业生态研究所,黑龙江,哈尔滨,150081 |
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| 基金项目： | 中国科学院学科前沿领域项目，国家科技计划项目 |
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| 摘 要： | 采用液体和固体两种培养方法繁殖异小杆线虫Heterorhabditis bacteriophora - 1（Hb—1），测定和计算不同方法得到的线虫产量、质量和效率。试验结果表明，TSYS液体培养中接种单菌线虫是非单菌线虫产量的2．1倍，效率是其3．5倍。TSYS液体培养基培养线虫的效率明显高于PSYG液体培养基。TSYS固体培养线虫的产量和效率是其液体培养的3．7倍。液体培养和固体培养线虫均在第17d开始收获。20d后调查，固体培养的线虫致病力和存活率稍高于液体培养。图5，参13。
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| 关 键 词： | 昆虫病原线虫 大量繁殖 离体 异小杆线虫 |
Technology for in Vitro Mass Production of Heterorhabditis bacteriophora -1 |
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| WANG Li-fang,LI Chun-jie,XU Yan-li.Technology for in Vitro Mass Production of Heterorhabditis bacteriophora -1[J].System Sciemces and Comprehensive Studies In Agriculture,2010,26(1):31-35. |
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| Authors: | WANG Li-fang LI Chun-jie XU Yan-li |
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| Institution: | 1. Northeast Institute of Geography and Agricultural Ecology/a>;Chinese Academy of Sciences/a>;Harbin 150081/a>;China/a>;2. Graduate University of Chinese Academy of Sciences/a>;Beijing 100049/a>;China |
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| Abstract: | In order to screen better medium and method,liquid and solid methods were used to produce Heterorhabditis bacteriophora - 1(Hb - 1). The results showed that the yield of inoculated mono-nematodes was 2. 1 times and the efficiency of that was 3. 5 times as inoculated surface sterile ones in TSYS liquid,and the efficiency of produced nematodes in TSYS was 1. 6 times as in PSYG. The efficiency and yeild of TSYS solid production was 3. 7 times as TSYS liquid. The nematodes produced by TSYS liquid and solid prod... |
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| Keywords: | entomopathogenic nematode mass production in vitro Heterorhabditis bacteriophora-1 |
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