菊三七根中化合物seneciphyllicacid和senecinicacid的含量测定

目的：采用HPLC法测定菊三七根部seneciphyllicacid和senecinicacid的含量．方法：建立HPLC法测定菊三七根部seneciphyllicacid和senecinicacid的含量．色谱柱为AcclaimC18色谱柱（4．6mm×250mm，5μm），流动相为甲醇-0．1％甲酸水（40：60，v·v-1），流速为1．0mL·min-1，柱温为30℃，紫外检测波长为220nm．线性范围：Seneciphyllicacid为0．1～0．8mg·mL-1（相关系数r=0．9997）；senecinicacid为0．1～1．5mg·mL。（相关系数r=0．9998）．结果：测得菊三七根部seneciphyllicacid和senecinicacid的含量分别为2．9613mg·g-1、0．9542mg·g-1；RSD分别为1．34％、1．28％；平均回收率为98．84％、99．16％，RSD为1．94％、1．51％．结论：该方法快速、简便、准确度高，可为开发利用菊三七提供研究基础．

Content Determination of Seneciphyllic Acid and Senecinic Acid in Roots of Gynura Japonica

The contents of seneciphyllic acid and senecinic acid in root of Gynura Japonica were determined by HPLC and the method of HPLC determination was established . The analysis was operated on a Acclaim C18 column（4.6 minx 250 mm,5μm）at 30℃ with mobile phase contained methanol-0. 1% formic acid water （40 ： 60,v·v-1）at a flow rate of 1 mL·min-1 followed by UV detection at 220 nm. The calibration curve of seneciphyllie acid was well linear over the concentration rang from 0.1 to 0.8 mg·mL-1 （r=0. 9997）;; the calibration curve of senecinic acid was well linear over the concentration rang from 0.1 to 1.5 mg·mL-1 （r= 0. 9998）. The contents of seneciphyllic acid and senecinic acid in root of Gynura Japonica are 2. 9613mg·g-1,0. 9542mg·g-1, respectively. The average recovery of seneciphyllic acid and senecinie acid are 98.84%, 99.16% with a RSD of 1.94%, 1.51%. This method is fast, simple and high accuracy and will provide tech-nology for the development and utilization of Gynura Japonica.