| 反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立 |
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| 引用本文： | 杨其伟,史国利,陈雅文,刘丛,陈德风.反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立[J].南开大学学报,1998(1). |
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| 作者姓名： | 杨其伟 史国利 陈雅文 刘丛 陈德风 |
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| 作者单位： | 南开大学分子生物学研究所!天津300071 |
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| 基金项目： | 国家自然科学基金!39480016 |
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| 摘 要： | 利用DNA体外重组技术将PARP基因cDNA部分序列反向克隆到真核表达载体pMAMneo上，构建成重组质粒pMAMneo－C1．4和pMAMneo－C0．3．将重组质粒pMAM－neo－C1．4转染HeLa细胞，经G418筛选，建成细胞系HeLa－C1．4－neo，Southern杂交结果表明，外源PARP基因cDNA部分序列已稳定整合到受体细胞基因组中，该细胞系的建立为研究聚ADP核糖基化作用在细胞内的功能打下了基础．
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| 关 键 词： | PARP基因 反义部分序列 DNA转染 转化细胞系 |
CONSTRUCTION OF ANTISENSE PARP cDNA RECOMBINANTS AND ESTABLISHMENT OF THE HeLa TRANSFORMANT AFTER ANTISENSE PLASMID INTRODUCING |
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| Yang Qiwei, Shi Guoli, Chen Yawen, Liu Cong, Chen Defeng.CONSTRUCTION OF ANTISENSE PARP cDNA RECOMBINANTS AND ESTABLISHMENT OF THE HeLa TRANSFORMANT AFTER ANTISENSE PLASMID INTRODUCING[J].Acta Scientiarum Naturalium University Nankaiensis,1998(1). |
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| Authors: | Yang Qiwei Shi Guoli Chen Yawen Liu Cong Chen Defeng |
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| Abstract: | The recombinants pMAMneo-C1. 4 and pMAMneo-C0. 3 were constructed by inserting varied partial length cDNAs of poly (ADP-ribose) polymerase gene into mammalian expressive vector pMAMneo respectively. The transformed cell line, HeLa-C1. 4-neo, capable of generating antisense RNA for poly (ADP-ribose) polymerase gene was obtained by introducing pMAMneo-C1. 4 plasmid into HeLa cells. Southern blot analysis showed that the exogenous partial cDNA for poly (ADP-ribose) polymerase had been integrated into the cell chromosomes. The established cell line laid a foundation for evaluating the biological roles of poly (ADP-ribosyl) action in eukaryotic cells. |
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| Keywords: | gene for poly (ADP-ribose) polymerase antisense partial sequence DNA transfection transformed cell line |
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