| 银杏叶提取物指纹图谱的研究与应用 |
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| 引用本文： | 陈凡,李奕雅,庄远红,王玉玲.银杏叶提取物指纹图谱的研究与应用[J].漳州师院学报,2010(1):92-95. |
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| 作者姓名： | 陈凡 李奕雅 庄远红 王玉玲 |
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| 作者单位： | 漳州师范学院生物科学与技术系,福建漳州363000 |
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| 基金项目： | 漳州师范学院科学研究资助项目（SK09021） |
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| 摘 要： | 采用HPLC法,对银杏叶的提取物进行了指纹图谱研究：使用Waters Nova-Pak C18（Φ3.9×150 mm,4μm）色谱柱,流动相为0.2%磷酸水溶液和乙腈,二元梯度洗脱,流速1.0 mL/min,柱温34℃,检测波长为310 nm和355 nm.利用该法对两种不同厂家生产的银杏叶提取物,以及自制银杏叶醇提物、水提物进行了检测,取得了满意的效果.该法操作简单、稳定性、精密度高,可作为各类银杏叶原料、中间产物及银杏叶提取物产品的质量控制依据.
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| 关 键 词： | 银杏叶 银杏叶提取物 指纹图谱 银杏叶黄酮 银杏酸 |
Study and Application of Fingerprints of Ginkgo biloba Extracts |
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| CHEN Fan,LI Yi-ya,ZHUANG Yuan-hong,WANG Yu-ling.Study and Application of Fingerprints of Ginkgo biloba Extracts[J].Journal of ZhangZhou Teachers College(Philosophy & Social Sciences),2010(1):92-95. |
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| Authors: | CHEN Fan LI Yi-ya ZHUANG Yuan-hong WANG Yu-ling |
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| Institution: | (Department of Biology Science and Technology,Zhangzhou Normal University,Zhangzhou,Fujian 363000,China) |
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| Abstract: | A HPLC method for the determination of fingerprints of Ginkgo biloba extracts was established.Waters Nova-Pak C18（Φ3.9×150 mm,4 μm） was used as analysis column and 0.2% H3PO4/acetonitrile as mobile phase at the flow rate of 1.0 mL/min with gradient elution.The column temperature was at 34 ℃,the wavelength of DAD detector was at 310 nm and 355 nm.This method was successfully applied to determine two kinds of EGb that from different manufacturer and the ethanol/water extracts of ginkgo leaves.This method was convenient,stable and repeatable.It can be applied to the quality control of ginkgo leaves,its extracts and relative products. |
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| Keywords: | ginkgo leaves Ginkgo biloba extracts fingerprint ginkgo flavornoids ginkgolic acids |
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