| SEDSS/异辛烷/水反胶束体系对Cyt-C的提取 |
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| 引用本文： | 周子彦,谢玉忠,杨艳杰,褚莹,吴子生. SEDSS/异辛烷/水反胶束体系对Cyt-C的提取[J]. 东北师大学报(自然科学版), 2001, 33(1): 48-51 |
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| 作者姓名： | 周子彦 谢玉忠 杨艳杰 褚莹 吴子生 |
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| 作者单位： | 延边大学理工学院化学系,;东北师范大学化学学院, |
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| 基金项目： | 国家自然科学基金资助项目(29673009) |
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| 摘 要： | 报道了在SEDSS／异辛烷／水反胶束体系中相转移提取细胞色素C的最佳条件。结果表明,在室温下,pH值为2－6,离子强度为0．07－0．25mol.L^-1及表面性剂的浓度大于60mmol.L^-1的条件下,提取率可达99％以上;pH=12.2离子强度为0．5mol.L^-1及乙醇体积分数为50％的条件下,反提取率可达57％,正提和反提时都无界面膜状物产生。
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| 关 键 词： | 丁二酸二(油酸乙二醇单酯)酯磺酸钠(SEDSS) 反胶束 提取 细胞色素C |
| 文章编号： | 1000-1832(2001)01-0048-04 |
| 修稿时间： | 2000-11-10 |
Extraction of cytochrome C with SEDSS/iso-octane/water reversed micellar system |
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| ZHOU Zi-Yan,XIE Yu-zhong,YANG Yan-Jie,CHU Ying,WU Zi-sheng. Extraction of cytochrome C with SEDSS/iso-octane/water reversed micellar system[J]. Journal of Northeast Normal University (Natural Science Edition), 2001, 33(1): 48-51 |
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| Authors: | ZHOU Zi-Yan XIE Yu-zhong YANG Yan-Jie CHU Ying WU Zi-sheng |
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| Abstract: | This paper reports the best condition of the phase transfer extraction of cytochrome C in SEDSS/iso-octane/water reverse micellar system. The results indicated that the extraction ratio is above 99% in the pH range 2 to 6, ionic strength equal 0.07 to 0.25 mol.L-1, concentration of surfactant is above 60 mmol.L-1, the back extraction ratio is above 57% in the pH is 12.2, ionic strength equal 0.5 mol.L-1,concentration of ethanol (Vethanol／Vwater) is 50%, no undissolved substance on the interface, and need not co-surfactant. |
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| Keywords: | sodium bis (monoglycololeoly) sulfuo-succinate (SEDSS) reversed micelle extraction Cyt-C. |
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