Inhibition of aldose reductase by dihydroflavonols inEngelhardtia chrysolepis and effects on other enzymes

Astilbin and neoastilbin, dihydroflavonol rhamnosides fromEngelhardtia chrysolepis, showed potent inhibition of lens aldose reductase. Kinetic analysis showed astilbin exhibited uncompetitive inhibition against bothdl-glyceraldehyde and NADPH. These taxifolin glycosides were selective inhibitors of aldose reductase with no inhibition of NADH oxidase.     

荭草花旗松素提取工艺优化

采用超声波预处理结合热回流提取的工艺提取荭草花旗松素,利用响应面分析法对提取工艺进行优化。结果表明,最佳提取工艺条件为：超声波处理时间20min、超声波处理温度50℃、超声波功率100W、乙醇体积分数55％、热回流提取温度87℃、料液比1：22（g·mL^-1）、热回流提取时间2h、提取次数2次,此条件下荭草花旗松素的提取超为3,56mg·g^-1。