Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S5n allel...     

日粮中不同Mn,Zn水平对雏鸡卵黄囊中Mn,Zn转移的影响

取自行孵化的新出壳ＡＡ公雏鸡５７６只分为两批分别用于Ｍｎ和Ｚｎ的试验，每批分为四组，每组随机地对应高Ｍｎ或高Ｚｎ、低Ｍｎ或低Ｚｎ，无Ｍｎ和Ｚｎ和标准Ｍｎ或Ｚｎ四种日粮水平的一种，于２，４，６，８，１２，１６，２４，３６，４８，７２，９６，１２０时龄每组分别取雏鸡６只测定卵黄囊中Ｍｎ或Ｚｎ的含量。测定结果进行方差分析和多重比较（ＳＡＳ）。试验表明，雏鸡出壳２小时内卵黄囊Ｍｎ和Ｚｎ的含量分别为０．１９     

腹腔镜疝囊高位结扎术治疗小儿斜疝156例临床分析

分析2002年10月至2008年3月应用小儿微型腹腔镜配合自行设计的带线雪橇钩针开展小儿腹股沟斜疝的腹腔镜疝囊高位结扎术156例,探讨应用小儿微型腹腔镜治疗小儿腹股沟斜疝的疗效、并发症及预防措施。结果表明,应用小儿微型腹腔镜治疗腹股沟斜疝比传统手术优越,具有手术时间短、创伤小、切口小、瘢痕小、复发率低、并发症少、患儿痛苦少、恢复快、住院时间短等优点,可同时发现及处理隐性内环口未闭,且在双侧腹股沟斜疝及复发性斜疝治疗上有明显优势,值得临床推广应用。     

树枝状聚合物PAMAM葛根素复合物的制备及肠吸收研究

制备树枝状聚合物G3,G4,G5 PAMAM葛根素复合物,研究其在大鼠不同肠段的吸收情况.采用大鼠离体外翻肠囊法、高效液相色谱法测定葛根素大鼠小肠吸收参数,以未加PAMAM组作为空白对照组,计算累积透过量及表观通透系数(Papp).葛根素在空肠及回肠段吸收明显,表观通透系数分别为(2.98±0.22)×10-6、(3.94±0.30)×10-6cm·s-1,PAMAM与葛根素形成复合物能增加葛根素大鼠小肠累积透过量及表观通透系数.其中G5 PAMAM对葛根素促进作用最为显著,葛根素空肠、回肠段表观通透系数分别增加了2.78、2.22倍(P＜0.05).PAMAM能有效促进葛根素大鼠小肠吸收.     

不同洗眼液对结膜囊冲洗效果的影响

目的:探讨用两种洗眼液进行结膜囊冲洗(简称洗眼)对眼部的影响效果。方法:将492人分为两组进行洗眼:A组304人用生理盐水进行洗眼2次;B组188人用蒸馏水进行洗眼2次,了解洗眼后的眼部不适和结膜充血情况。结果:两组间眼部不适和结膜充血有显著性。结论:用生理盐水洗眼较用蒸馏水洗眼可减轻眼部不适及结膜充血。     

Positional variation of antipodal cells in polyembryonic rice ApⅢ before and after fertilization

The variation in the position of antipodal cells before and after fertilization in polyembryonic rice strain ApⅢ was studied by using conventional sectioning technique. It was shown that initially the three antipodal cells lie at the chalazal end of the young embryo sacs. Along with the development of embryo sac, the antipodal cells proliferate into a multicellular structure containing up to about 20 cells. Most of the cells migrate to its dorsal side when the embryo sacs turn into mature. In a few embryo sacs the antipodal cells are situated in the positions adjacent to the egg apparatus at the micropylar end, while in other sacs, they spread from the midst of the embryo sac to the micropylar end clinging to the nucellar tissue. Furthermore, it is reported for the first time that cells of some egg apparatus or proembryos disorganize when antipodal cells lie at the micropylar end, indicating that the function of the antipodal cells may vary during the embryogenesis in rice ApⅢ.     

漂白粉对三角帆蚌外套膜粘液细胞及珍珠囊细胞的影响

研究了不同浓度的漂白粉（0.5mg/L,1.0mg/L,1.5mg/L)处理后对三角帆蚌的影响,结果表明各浓度漂白粉对外套膜和珍珠囊细胞的超微结构均有不同程序损害,表现为粗面内质网脱颗粒,网腔扩张,线粒体基质电子密度改变等,其中1．5mg/L漂白粉的影响最为严重,除发生上述变化外,外套膜内表皮粘液细胞中硫酸化粘液减少,酸性粘液增加,外表皮细胞中性粘液几乎消失,该浓度组的结缔组织中钙球体的数量也明显高于其它各组。     

环境钙浓度对淡水育珠蚌外套膜及珍珠囊钙代谢的影响

作者采用多种组织化学方法及等离子光谱技术,研究了不同环境钙浓度对淡水育珠蚌钙代谢的影响。发现与对照组（钙浓度约１０ｇ／ｍ＾３）相比,较高钙浓度（２０～３０／ｍ＾３）条件下三角帆蚌外套膜组织钙含量增加,外套膜内外表皮和珍珠囊上皮细胞的分泌活动旺盛;与之相反,高钙浓度条件下（３０ｇ／ｍ＾３以上）,蚌外套膜组织钙含量降低,外套膜内外表皮和珍珠囊上皮细胞的分泌活动下降。这表明环境中适宜的钙浓度可促进蚌对钙     

沙冬青成熟胚囊助细胞的超微结构观察

本文对沙冬青（Ａｍｍｏｐｉｐｔａｎｔｈｕｓｍｏｎｇｏｌｉｃｕｓ）成熟胚囊助细胞的超微结构进行了观察，结果如下：１）开花前一天助细胞珠孔端有明显的丝状器，并在其附近的细胞质中有许多管状内质网，核位于珠孔端，细胞器主要集中在核周围和合点端细胞质中，两助细胞超微结构一致。２）开花当天，助细胞合点端细胞壁消失，近珠柄侧的助细胞中细胞器变得稀少，内质网膜和质体膜变得模糊不清，而远珠柄例的助细胞中细胞器发达，此时，两助细胞在超微结构上显示出明显的差异，表明与其功能、退化和花粉管进入有关。