食荚型豌豆组织培养和植株再生研究

以豌豆子叶、茎、真叶为材料进行外植体筛选及组织培养研究,试验结果表明:豌豆带节茎段是最佳试验材料;在MS附加1 mg/L BA和1 mg/L NAA培养基中,愈伤组织诱导率达100%,愈伤组织在分化培养基中未分化出芽;带节茎段在MS附加2～3 mg/L BA和0.1 mg/L NAA培养基中,腋芽和丛生芽产生率达100%,芽增殖系数3以上;不定芽在MS附加3 mg/L NAA上生根率86%;试管苗移栽成活率85%左右.     

Chronic mercury vapor poisoning of aphids

Summary Exposure of green peach aphids,Myzus persicae (Sulz.), to an atmosphere containing mercury vapor resulted in a curtailment ofembryogenesis and larviposition by adults, and in the development by larvae and adults of a cuticular darkening of their legs, head capsule, antennae, cornicles and cauda. Mortality of affected larvae resulted from molting difficulties, particularly by last-instar alatiform female and male larvae. Greenbugs,Schizaphis graminum (Rond.), and pea aphids,Acyrthosiphon pisum (Harr.), responded to mercury vapor exposure in similar ways.     

超高产豌豆新品种引种及配套栽培技术研究

通过对豌豆新品种引种及栽培配套技术研究,成功地筛选出超高产豌豆新品种９０－ＰＥ－１０。该品种为法国埃松省种业集团新育成的优良品种,主要特征特性是：高产,比推广品种（中豌５号）增产７０％以上;优质,粗蛋白含量比对照品种高２．７％,提高了１４．９％;抗病虫,抗褐斑病、白粉病,由于该品种叶片蜡被较厚,具有一定抗潜叶蝇能力;抗侄伏,卷须发达,并在株间相互缠绕,使个体联结成群体,成棚架结构;适宜芽菜生产,由于卷须极发达,符合芽菜中龙须菜生产要求。     

浅谈改良CTAB法提取豌豆基因组DNA

通过改良CTAB法从豌豆（Pisum sativum）嫩叶中提取了基因组DNA,并进行了紫外分光光度计和琼脂糖凝胶电泳分析,结果表明,得到的DNA一级结构较完整,杂质较少,浓度也较高,可进一步用于PCR（Polymerase chain reaction）和酶切等分子生物学实验。     

基于卫星遥感数据的库尔勒香梨识别提取方法研究

针对目前国内利用卫星遥感数据的光谱特征及其植被指数对香梨识别提取方法鲜有研究的现状,探讨研究了Landsat8_OLI数据对不同树龄香梨的识别提取方法和实际应用。以库尔勒市中部的英下乡和沙依东园艺场为试验区域。采用多时相Landsat8_OLI数据。通过分析试验区内典型地物的光谱特征和植被指数的变化规律以及作物物候历的不同,选择不同树龄香梨树林遥感识别的最佳时相,利用决策树方法构建了不同树龄香梨树林的识别提取模型,实现了香梨种植面积的有效提取。结果表明:1香梨成林(种植5a以上的香梨树林)遥感提取效果较为满意,精度为93.51%,而香梨幼林由于受多种因素的影响,其提取精度只有78.79%,有待进一步研究;2 Landsat8_OLI数据具有较高的时空分辨率,是遥感识别提取库尔勒香梨的潜力数据源。     

豌豆新品种宝峰3号的营养品质及产量构成因素

宝峰3号是以中豌5号为母本、90-PE-10为父本杂交选育而成的豌豆新品种,为确定其营养品质及产量构成因素,对宝峰3号进行了试验研究.结果表明：宝峰3号品质良好;产量高,平均比对照中豌6号增产42.6%.实现了超高产（单位面积增产量42.6%）、优质相结合的指标.     

干旱条件下两种山黧豆气孔特性及种子ODAP,粗蛋白和淀粉积累的研究

对干旱条件下永寿山黧豆(YS)和定西山黧豆(DX)的气孔特性、光合特性和种子物质的积累进行了研究。结果表明，干旱使两种山黧豆气孔密度(SD)显著升高，同时显著减小气孔开度(SA)，SA在30％以上的气孔所占的比例显著减小，而气孔阻力(r)却显著增加；干旱条件下，两种山黧豆的光合速率(Pn)和蒸腾速率(E)均较正常水分条件下的小，而叶片水平水分利用效率(WUE)却显著上升；干旱使两种山黧豆种子千粒重降低，而种子ODAP，粗蛋白和淀粉含量均有一定程度增加，相比之下，干旱更严重地影响了YS的SD，SA，r，Pn，E以及种子ODAP含量，而更显著地提高DX的WUE和种子粗蛋白、淀粉含量。分析认为，干旱通过影响气孔密度和开度而影响光合速率蒸腾速率和水分利用效率；干旱也增加了气孔阻力，而气孔阻力的增加大幅度地减小了蒸腾速率；光合速率、蒸腾速率和水分利用效率的变化最终影响种子千粒重以及种子ODAP，粗蛋白和淀粉等物质的积累；DX可能较耐旱，水分亏缺对YS的影响较DX大。     

The effects of microfilament and microtubule inhibitors and periodic electrical impulses on phloem transport in pea seedling

In phloem transport, whether protoplasmic activity participates in assisting sap flow in sieve element-companion cell complcx hils long been in debate. The prcsent investigation assumcd microfilament (MF) and microtubule (MT), the two constituents of the protoplasmic cytoskeleton, as motive force, and employed germinating pea seedling suspended in moist chamber as experinental material: thc seed being the source; the elongating root, the sink.¹⁴C-labeled sucrose was added to the seed as indicator. The amount of sap transported from source to sink was measured by the increase in root elongation. The transport phloem was within the cylinder of the peclcd root in thc middle. The exposed cylinder was treated with MF inhibitor (cytochalasin B), or microtubule inhibitor (amiphosmethyl). Results showed that the sap influx into the elongating root. and the¹⁴C activity as well, was reduced by abut one half in treatrrrent with cytochaliisin B, and much less by amiphos-methyl treatment. Similar effect was shown in clcctrical impulse Ireatnimt, which scems to disrupt the MF and MT configuration.     

豌豆壳沼气发酵潜力的研究

实验以豌豆壳为原料,采用批量发酵工艺,进行发酵产沼气实验,实验结果表明,豌豆壳是一种较好的沼气发酵原料,其沼气发酵潜力为208ml/gTS,220ml/gVS。