EBJ-120TP型掘进机的推广使用及改进

介绍了EBJ—120TP型掘进机在杜儿坪矿的使用情况,提出了EBJ—120TP型掘进机的改进建议及维护保养措施。     

基于VB与MO的120急救系统的设计与开发

120急救在现代城市中扮演着越来越重要的角色,快速、准确、高效的指挥决策和调度是城市急救系统实现的关键.系统从功能和技术两方面入手,在分析整合平顶山市现有120急救系统的基础上引入GIS、GPS和GSM技术,利用组件mapobjects,结合visual basic开发语言,实现对数据的空间分析和管理,建立了基于GIS的城市120急救系统,实现了信息的查询分析、最佳路径分析以及缓冲区分析功能.     

EIAV强毒株和疫苗株gp45的纯化及结晶比较

EIAV (equine infectious anemia virus)疫苗是世界唯一成功的慢病毒疫苗.通过对强毒株和疫苗株EIAV相关蛋白结构的比较研究,可以促进对其致弱过程及免疫机制的了解.gp45 (glycoprotein 45)是EIAV介导与宿主膜融合过程的关键蛋白.以EIAV强毒株LN40和疫苗株FDD...     

含抗HIV广谱中和抗体2F5、4E10靶基因载体的构建及鉴定

目的:构建含HIV gp120,gp41序列中广谱中和抗体2F5,4E10作用靶基因的载体并进行鉴定,为后期重组载体表达产物诱导产生中和抗体及抗HIV亚单位疫苗的研究奠定基础.方法:根据NCBI中HIV gp120,gp41基因序列中可与2F5、4E10结合的区域设计引物并进行PCR反应,将PCR得到的目的片段插入到载体pET28a中,对重组载体进行PCR鉴定、酶切鉴定及DNA测序.结果:PCR鉴定、酶切鉴定及DNA测序结果证实重组载体构建成功.结论:成功构建了含HIV gp120,gp41序列中广谱中和抗体2F5,4E10作用靶基因的载体.     

SoC在数控直流电流源设计中的应用

设计了一种基于SoC芯片C8051F120的数控直流电流源,该系统以闭环负反馈放大电路为恒流源模块,C8051F120为控制核心,利用SoC片上资源DAC和ADC,实现了数控输出步进为1mA、范围为20mA-2000mA的电流,设置和实际输出的电流都由LCD显示,软件采用线性补偿算法提高控制精度。该设计最大限度地降低了系统硬件电路和软件编程的复杂度,电源稳流效果好,控制精度高,系统可靠性较高。     

人免疫缺陷病毒跨膜蛋白gp41在大肠杆菌中的表达

为研究廉价的HIV血清学诊断系统,实现检测试剂国产化,用原核pET系统表达HIV跨膜蛋白gp41.研究发现,全长及缺失C端1／3的gp41在E.coli中均不能有效表达,仅保留N端1／3的gp41(包含gp41主要抗原决定簇,594－613氨基酸）有一定量的表达;通过金属螯合层析,产物得到部分纯化,Western blot显示产物有很好的反应原性,推测gp41在E.coli中较低表达量可能与mRNA的二级结构有关。     

负120°电压矢量参与调节的直接转矩控制系统

在直接转矩控制中以磁链偏差、转矩偏差以及磁链位置来合理的选择电压矢量，力求形成逼近圆形的多边形磁链，并且在转速给定突然变小时加入负120°电压矢量，以帮助零电压矢量的调节，在电机转速要求为零且要有电磁转矩输出时该电压矢量显得尤为重要，这样的系统在matlab/simulink中得以实现，取得较好的结果。     

一种高效免疫佐剂热休克蛋白gp96的酸性区和两侧中间区对其N端构象及多肽结合能力的调节作用

gp96(glucose-regulated protein,GRP94)是热休克蛋白90家族(HSP90 family)中的一员.由于其在维持内质网蛋白中间过渡态的空间构型以及免疫反应中有重要作用,因此近年来被作为分子伴侣蛋白和免疫佐剂引起广泛关注,但是关于gp96生物功能的彻底阐明还将依赖于对其结构的深入研究.在试验中我们构建了不同的gp96片段,旨在寻找gp96的重要功能区.通过凝胶分子筛和EGS交联实验,发现gp96中间酸性区可以调节gp96 N端的寡聚化状态;而固相ELISA实验显示,包含中间酸性区的N端重组蛋白与肽的结合有高度饱和性,这些结果表明中间酸性区对gp96的寡聚化以及多肽结合能力有内在的调节作用.这一研究对gp96生物功能的阐明以及临床应用提供了理论基础.     

Windows Vista下的虚拟光驱软件

Windows Vista逐渐成为主流的计算机操作系统。Vista最大的问题是软件的兼容性问题,一些老版本软件像虚拟光驱软件等无法在Vista下安装或运行。本文介绍了几种可以在Vista下运行的虚拟光驱软件,如DAEMON Tools、PowerISO、Alcohol 120%、WinMount等,并作了总结与评论。     

Molecular motions and conformational transition between different conformational states of HIV-1 gp 120 envelope glycoprotein

The HIV-1 gp120 exterior envelope glycoprotein undergoes a series of conformational rearrangements while sequentially interacting with the receptor CD4 and coreceptor CCR5 or CXCR4 on the surface of host cells to initiate virus entry. Both the crystal structures of the HIV-1 gp120 core bound by the CD4 and antigen 17b and the SIV gp120 core pre-bound by CD4 are known. Despite the wealth of knowledge on these static snapshots of molecular conformations,the details of molecular motions involved in conformational transition that are crucial to intervention remain elusive. We presented comprehensive comparative analyses of the dynamics behaviors of the gp120 in its CD4-complexed,CD4-free and CD4-unliganded states based on the homology models with modeled V3 and V4 loops by means of CONCOORD computer simulation to generate ensembles of feasible protein structures that were sub-sequently analysed by essential dynamics analyses to identify preferred concerted motions. The re-vealed collective fluctuations are dominated by complex modes of combinational motions of the rota-tion/twisting,flexing/closure,and shortness/elongation between or within the inner,outer,and bridg-ing-sheet domains,and these modes are related to the CD4 association and HIV neutralization avoid-ance. Further essential subspace overlap analyses were performed to quantitatively distinguish the preference for conformational transitions between the three states,revealing that the unliganded gp120 has a greater potential to translate its conformation into the conformational state adopted by the CD4-complexed gp120 than by the CD4-free gp120,whereas the CD4-free gp120 has a greater potential to translate its conformation into the unliganded state than the CD4-complexed gp120 does. These dynamics data of gp120 in its different conformations are helpful in understanding the relationship between the molecular motion/conformational transition and the function of gp120,and in gp120-structure-based subunit vaccine design.