5-羟色胺对大鼠肝细胞DNA合成增强作用的研究

目的：调查诱导或增强肝细胞ＤＮＡ合成因素。方法：；从成年大鼠肝脏分离肝细胞，在含＾３Ｈ－胸苷的培养液中培养，向含或不含表皮生长因子（ＥＧＦ）和胰岛素的肝细胞培养加入不同浓度的５－羟色胺（５－ＨＴ）以观察对ＤＮＡ合成的影响。在部分含５－ＨＴ的培养中加入５－ＨＴＨ２受体拮抗物酮舍林以检查Ｈ２受体的参与。ＤＮＡ合成以＾３Ｈ－胸苷掺入肝细胞ＤＮＡ的量用液体闪烁计数法测量。结果５－ＨＴ单独不能刺激ＤＮＡ合成     

注射用促肝细胞生长素中组胺类物质检查和降压物质检查的比较研究

目的 探讨注射用促肝细胞生长素安全性检查中,以组胺类物质检查法替代降压物质检查法的可能性。方法 按照《中国药典》2015年版中降压物质检查和组胺类物质检查方法的规定,分别对3批注射用促肝细胞生长素进行降压物质检查和组胺类物质检查,比较两种检查方法结果的异同。结果 1.根据药典相关判定标准,3批注射用促肝细胞生长素降压物质检查和组胺类物质检查结果一致,均符合规定。2. 降压物质检查中3批注射用促肝细胞生长素均引起猫血压明显下降,结果呈阳性;3批供试品均未引起豚鼠离体回肠收缩,结果呈阴性。结论 注射用促肝细胞生长素在组胺类物质含量符合要求时也可能引起动物血压明显下降,体外实验结果不能简单扩推至体内实验。     

肝细胞原代培养研究综述

对国内外肝细胞培养研究作出综述.首先介绍了分离肝细胞的各种方法及各自的优缺点, 重点对各种酶分离技术进行了比较.其次阐述了肝细胞的纯化方法和生物学性状检测,最后综述了 影响肝细胞体外培养的因素.     

The use of primary cultures of adult rat hepatocytes to study induction of enzymes and DNA synthesis: Effect of nafenopin and electroporation

Summary Primary cultures of adult rat hepatocytes maintained in a well-differentiated state, in a chemically defined medium containing 2% DMSO, have been utilized to study the effect of non-mutagenic hepatocarcinogens such as the peroxisome proliferator nafenopin. The parameters chosen in this in vitro system were those that paralleled the major in vivo effects of nafenopin on the liver, mainly: the proliferation of the endoplasmic reticulum and induction of cytochrome P-452, the proliferation of the peroxisome compartment and the induction of cyanide-insensitive -oxidation of fatty acids and the stimulation of liver growth as measured by the DNA synthetic activity of the hepatocytes.In this review, we also describe the morphology of hepatocyte cultures prepared from previously electroporated hepatocytes and the potential for the use of electroporation to introduce growth related genes into hepatocyte cells to study the mechanisms of hepatocyte growth at the molecular level. In addition we describe the formation of endoplasmic reticulum whorls in these cultures as a consequence of nafenopin treatment. Whorl formation by hepatotrophic chemicals has been previously shown to occur in vivo; in this report, it is described for the first time in vitro.     

复方山楂煎剂对非酒精性脂肪肝大鼠模型的影响

目的 研究复方山楂煎剂对非酒精性脂肪肝的治疗作用及其作用机制。方法 将60只Wistar大鼠随机分 为空白对照组、模型对照组、复方山楂煎剂(低剂量14. 85 g生药/kg、中剂量29.7 g生药/kg、高剂量59.4 g生药/ kg)组,脂必妥对照组,除空白对照组外其余各组采用高脂饲料喂养大鼠8周,造模后空白对照组、模型对照组灌胃给予蒸馏水,其余各组灌胃给予相应药物,治疗6周。实验结束后,检测各组大鼠血清丙二醛(MDA)、超氧化物歧 化酶(SOD)、谷丙氨酸转氨酶(ALT)、谷草转氨酶(AST)以及肝组织中的甘油三酯(TG)和肝体比,并采用HE染色 观察肝组织脂肪变性程度,采用免疫组化法检测肝组织中肝细胞生长因子(HGF)及结缔组织生长因子(CTGF)的 表达。结果 复方山楂煎剂可显著降低脂肪肝模型大鼠肝体比、血清MDA、ALT以及肝脏TG含量(P<0.05或P< 0.01)和结缔组织生长因子(CTGF)在脂肪肝模型大鼠肝组织中的表达(P<0.05)。复方山楂煎剂中剂量组、高剂量组可显著降低脂肪肝模型大鼠血清AST含量(P<0. 05或P<0. 01)、显著提高脂肪肝模型大鼠血清SOD的活性 (P<0. 05或P<0.01)和显著降低肝细胞生长因子(HGF)在脂肪肝模型大鼠肝组织中的表达(P <0.05)。复方山楂 煎剂可显著降低高脂饮食诱发的肝细胞脂肪变性程度。结论复方山楂煎剂可有效地用于非酒精性脂肪肝的治疗。