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1.
Summary Normal human spermatozoa were demonstrated by dot immunoblot analysis and immunohistochemistry to possess transglutaminase (TGase). The immunological identification of spermatozoal TGase is consistent with reports by others of its biochemical identification and suggested role in sperm motility,and provides, in view of the immunoregulatory properties of seminal plasma TGase, presumptive identification of a means whereby spermatozoa, under normal physiological conditions, may possibly be protected from immunological attack within the female reproductive tract. 相似文献
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低功率密度微波辐射对人细胞的非热效应 总被引:2,自引:0,他引:2
采用频率为2450MHz,功率密度为3,5和7mW/cm^2的微波照射人精子1h,发现3mW/cm^2和7mW/cm^2的微波对人精子的活动度、存活率、畸形率和穿卵率没有任何影响,而5mW/cm^2的微波对从精子的活动度、存活率、畸形率及其穿卵率有显著的影响。实现表明,微波的非热效应不仅存在,且在5mW/cm^2附近有明显的功率密度窗效应。 相似文献
3.
LIU Jianzhong~ ZHANG Yiling~ . College of Chemical Engineering Technology Wuhan University of Science Technology Wuhan Hubei China . College of Animal Science Technology Northwestern University of Science Technology in Agriculture Forest Yangling Shannxi China 《武汉大学学报:自然科学英文版》2006,11(2):447-450
0Introduction Afterthecarryingoutofopen doorpolicies,espe ciallyinthelatesttenyears,livestockandpoul try’sraisingbusinessdevelopedveryfastinChina,whichhelpeduscompletelyshrugofftheembarrassed situationofsevereshortagesofanimalproteininthepastlongperiodandgreatlyexcitedandencouraged us.Meanwhilewemustclearlyrealizethepotentialdangersbehindthepresentprosperities.Ourbreeds arebecominglessandless,manynativebreedswithlowperformancedisappearedcompletely,thetendency oftheirextinctionisincreasinginth… 相似文献
4.
To study the relations between male infertility and centrosome protein (centrin) and the functions of centrin in spermatogenesis,
the matured spermatozoa of 10 normal male people and 18 male infertility patients were stained by immunofluorescence labeling
antibody against centrin. The results showed that two fluorescence signal dots appeared in the normal male spermatozoa and
were located at the base of flagellum. They are proximal centriole and distal centriole. However, in some spermatozoa of the
male infertility, centrin protein was located abnormally at the base of flagellum and its staining signals were spread, the
normal proximal and distal centrioles were confused and could not be recognized separately. These results suggest that abnormality
of centrosome protein may be related to male infertility. This discovery may be used as a marker of abnormal sperm and male
infertility. 相似文献
5.
瓦氏黄颡鱼精巢发育及精子生物学研究 总被引:1,自引:0,他引:1
杨家云 《西南师范大学学报(自然科学版)》2005,30(4):719-724
瓦氏黄颡鱼精巢呈栉状分支,每一分支内部结构为叶型,精巢小叶由许多壶腹状的精小囊组成,精巢发育分为精原细胞增殖期、精母细胞生长期、精母细胞成熟期、精子细胞变态期、精子成熟期和精子退化吸收期,精子的发生和形成经历了精原细胞、精母细胞、精子细胞到成熟精子的一系列过程,精巢后1/3的精巢尾区由上皮细胞构成,既无生精功能也无贮精作用,成熟精子在0.45%~0.55%盐溶液和pH7.5的弱碱性的自然河水环境中保持的活性最好,并具有相当长的寿命。 相似文献
6.
以荧光探针Hoechst33342(Ho33342)特异性标记大白鼠精子和肝细胞DNA,用科学冷却型CCD显微荧光图像系统获取单个细胞的荧光图像,再以图像处理软件“Image-Pro PLus”,分析测量各细胞的DNA相对含量和细胞核的相对面积。实验数据表明:鼠肝细胞的DNA相对含量分别为精子的DNA相对含量的2,4,8倍,符合生物学的规律,说明这套系统的定量结果是可靠的。 相似文献
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8.
M. Murakami T. Nishida S. Iwanaga M. Shiromoto 《Cellular and molecular life sciences : CMLS》1984,40(9):958-960
Summary Scanning and transmission electron microscopic observations have been made in the terminal region of the vas deferens of the cat, with emphasis on the occurrence of spermiophagy. The present study has revealed that epithelial cells as well as luminal macrophages are extensively and actively involved in phagocytosis of spermatozoa. The mechanism of the spermiophagy is discussed, in relation to a possible role of the epithelial cells, as one function of the vas deferens. 相似文献
9.
Summary Observation by electron and phase-contrast microscopy demonstrated that inBombyx mori the dissociation of eupyrene bundles, apparently through digestion of the prostatic secretion or an endopeptidase, was promoted by the vigorously flagellating movement of apyrene spermatozoa in the spermatophore. 相似文献
10.
An animal unable to synthesize ascorbic acid uniquely minicks human and non-human primates. Therefore, in this study we used the rainbow trout, a teleost fish, as the model animal to study the importance of dietary ascorbic acid on the fertilizing ability of sperm. A high concentration of ascorbic acid in semen plays a key role in maintaining the genetic integrity of sperm cells, by preventing oxidative damage to sperm DNA. This study will show that the concentration of asorbic acid in seminal plasma refelcts the dietary fed either an ascorbate-free diet (from 4.74±0.9 to 0.16±0.08 g ml–1) or an ascorbate-rich diet (from 37.9±4.7 to 17.7± 3.2 g ml–1) during the sperimnation season. The relationship between ascrobate status and fertility was studied in six groups of fish fed graded levels of ascorbic acid, which sperimated over a 150-day-period. Sperm from individual males was used to fertilize several batches of eggs. When the seminal plasma ascorbate concentration decreased to 7.3 g ml–1 a significant decrease of fertilization rate and the hatching rate of embryos resulted. This is the first evidence that dietary ascorbate level directly affected sperm quality and influenced male fertility in a scruvy-prone vertebrate. 相似文献