Exendin-4螺旋结构对生物活性及稳定性的影响

针对Exendin-4的螺旋结构特点, 采用去掉螺旋序列、 用3个连续Ala替换螺旋以及Gln13替换为Tyr13的方式对N端α-螺旋及C端α-螺旋进行改造, 设计出4个结构模拟物. 通过圆二光谱和荧光光谱进行结构分析, 结合模拟物生物活性实验, 分析Exendin-4螺旋结构与生物活性的关系. 结果表明：  Exendin-4的N端螺旋比C端螺旋对生物活性影响更大； 在抗二肽基肽酶（DPPⅣ酶）的稳定性实验中, C端螺旋比N端螺旋具有更好维系Exendin-4稳定性的作用； 螺旋结构中Tyr13可作为提高Exendin-4生物活性的重要优化位点.     

一类具有饱和发生率和心理作用的随机SIR传染病模型

考虑一类受环境噪声影响, 具有饱和发生率和心理作用的随机SIR传染病模型. 通过构造Lyapunov函数并利用Ito公式, 得到该模型正解的全局存在唯一性, 并证明： 当随机基本再生数R*≤1时, 无病平衡点是随机渐近稳定的, 此时疾病将灭绝； 当R*>1时, 疾病将随机持续下去. 数值模拟结果验证了理论结果的正确性.     

循环作用对马蹄寺石窟群岩体性能的影响

马蹄寺石窟群地处河西走廊中部,气候条件十分恶劣。由于常年受到温度、湿度、酸碱盐等作用的影响,石窟岩体性能出现了不同程度的劣化现象。通过开展室内模拟试验,研究以上多种恶劣环境的循环作用对于岩体抗压强度、波速、质量的影响规律,同时使用X射线衍射仪(X-ray diffraction,XRD)、X射线荧光光谱(X-ray fluorescence,XRF)和扫描电子显微镜(scanning electron microscope,SEM)对循环作用过程中样品的矿物组分、元素含量和微观形貌进行分析研究。研究结果表明,循环作用使样品中的长石类矿物和方解石含量降低,黏土类矿物含量增加,胶结程度下降,孔隙逐渐发展成为横向微裂隙,导致样品的性能出现不同程度的劣化现象。循环作用对岩体性能的影响程度从大到小依次为冻融循环、耐盐循环、耐碱循环、耐酸循环和温湿循环。     

Cellular internalization of proinsulin C-peptide

Proinsulin C-peptide is known to bind specifically to cell membranes and to exert intracellular effects, but whether it is internalized in target cells is unknown. In this study, using confocal microscopy and immunostained or rhodamine-labeled peptide, we show that C-peptide is internalized and localized to the cytosol of Swiss 3T3 and HEK-293 cells. In addition, transport into nuclei was found using the labeled peptide. The internalization was followed at 37°C for up to 1 h, and was reduced at 4°C and after preincubation with pertussis toxin. Hence, it is concluded to occur via an energy-dependent, pertussis toxin-sensitive mechanism and without detectable degradation within the experimental time course. Surface plasmon resonance measurements demonstrated binding of HEK-293 cell extract components to C-peptide, and subsequent elution of bound material revealed the components to be intracellular proteins. The identification of C-peptide cellular internalization, intracellular binding proteins, absence of rapid subsequent C-peptide degradation and apparent nuclear internalization support a maintained activity similar to that of an intracrine peptide hormone. Hence, the data suggest the possibility of one further C-peptide site of action. Received 31 October 2006; received after revision 27 December 2006; accepted 30 December 2006     

台湾地区选举后群众运动的攻防策略

以 2004年台湾岛内由于选举而引发的大规模群众抗议活动为样本, 总结和分析泛蓝泛绿双方的主要攻防策略, 并在此基础上, 就运动的手段和策略等问题, 对群众运动的组织提出某些规范性意见。     

Maxwell—Stefan方程在多孔结构内传质方面的应用

介绍了颗粒间扩散及颗粒内的主体扩散、努森扩散和表面扩散的基本原理，描述了Maxwell-Stefan方程在既有主体扩散又有努森扩散的过程、表面扩散过程以及三种扩散形式都有的过程中的多孔结构内传质方面的应用．     

关于喷水室中热湿交换效率的研究

本文探讨了喷水室中的热湿交换机理,导出了反映不同换热效率之间的关系式,从中可知,η_1,η_2和X值是相互有关联的。利用本文导出的公式,可将不同作者通过实验所整理成的某一热湿交换效率值,变挟成其它换热效半值,以供从事空调设计和运行的人员使用。     

甲状腺肿块的高频彩色多普勒超声诊断

目的 :探讨高频彩色多普勒超声检查对甲状腺肿块的诊断价值。方法 :对 48例甲状腺包块患者进行二维及彩色多普勒血流显像超声检查。结果 :48例甲状腺包块患者共检出肿块 67个 ,超声检查与病理对照除 3例误诊外 ,其余 45例与病理结果包括肿块部位、数目均符合 ,符合率 90 % ( 42 / 45 )。结论 :高频彩色多普勒超声检查对甲状腺肿块具有较高的诊断价值 ,可为临床提供有价值的诊断依据     

Analysis of Pyrolysates for Phenol Formaldehyde Resin by Py-GC/MS

Pyrolysis of phenol formaldehyde resin has been investigated by Pyrolysis Gas Chromatography-Mass Spectroscopy at the different temperatures from 500℃ to 750℃. Its composition of pyrclysates has been analyzed. Several compounds, especially benzene, toluene, p-xylene could only be formed above 500-550℃. Howerver, peak intensities for some pbend derivatives were decreased at the higher temperature. During pyrolysis, for thermo-setting phenol formaldehyde resins, polymeric chain scissions take place as a successive removal of the monomer units from the polymeric chain. The chain scissions are followed by secondary reactions, which leads to a variety of compounds. Addition reactions can also take place among the double-bond compounds during pyrolysis.