大直径长钻孔桩孔底压浆技术的探讨

分析了某工程桩底缺陷形成的原因,介绍了桩底补强过程中的造孔、清淤、压浆及验证的具体做法。     

Improving the thermal stability of lactate oxidase by directed evolution

Lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. Increasing the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. We have previously obtained a variant of lactate oxidase from Aerococcus viridans with two mutations (E160G/V198I) that is significantly more thermostable than the wild-type enzyme. Here we have attempted to further improve the thermostability of E160G/V198I lactate oxidase using directed evolution. We made a mutant lactate oxidase gene library by applying error-prone PCR and DNA shuffling, and screened for thermostable mutant lactate oxidase using a plate-based assay. After three rounds of screening we obtained a thermostable mutant lactate oxidase, which has six mutations (E160G/V198I/G36S/T103S/A232S/F277Y). The half-life of this lactate oxidase at 70 °C was about 2 times that of E160G/V198I and about 36 times that of the wild-type enzyme. The amino acid mutation process suggests that the combined neutral mutations are important in protein evolution. Received 15 September 2006; received after revision 21 October 2006; accepted 2 November 2006     

表面纤维直径与羊毛机织精纺面料的刺痒感

通过前臂试验对26种轻薄型羊毛机织精纺面料和7种其他纤维的轻薄型机织面料的刺痒感进行了比较研究,并用显微镜对部分轻薄型羊毛机织面料的表面纤维分布进行了研究。结果表明多数毛织物在温度为(24±1)℃、相对湿度为(65±5)％的条件下存在刺痒感,并且毛织物的刺痒感与其表面纤维的平面直径及直径大于26μm的表面纤维根数密切相关。     

凸六边形的相对长边与相对短边

就Doliwaka和Lassak提出的凸n－边形的相对边问题，讨论n＝6的情形，将凸六边形嵌入到平等四边形内（边可重叠），利用对应边的边长比的关系可证任意凸六边形均有相对长边，有平行对边的凸六边形必有相对短边。     

2003版建筑给排水规范学习与设计体会

就新规范中住宅给水管管径、引入管阀门设置、水箱进水管和溢水管设置、水箱水位控制、通气管系统设计及地漏的设置等方面谈了作者学习《建筑给水排水设计规范》(GB50015-2003)的体会。     

基于符号定向图(SDG)深层知识模型的定性仿真

定性仿真是系统仿真技术中的重要研究和应用方向，同时也是人工智能技术的一个重要方面。近年来，随着现代计算机技术的日新月异，使得基于符号定向图(SDG)深层知识模型的定性仿真研究和应用取得了显著进展。SDG模型能够表达复杂的因果关系，具有包容大规模潜在信息的能力，因此特别适合于过程系统危险评价(PHA)、危险识别与故障诊断。SDG模型结构简单，易于建立和修改。然而高质量的SDG模型必须采用经验数据和定量动态仿真检验和校正。采用SDG模型进行定性仿真的关键技术是高效双向推理“引擎”，由计算机软件自动完成。目前，在计算机辅助过程系统危险评价方面，SDG是最有效的方法；在过程系统故障诊断方面，其完备性较好，但诊断分辨率尚待提高。今后的研究应解决：定性仿真和定量仿真相结合；SDG自动建模；通用推理软件平台；大系统高分辨率故障诊断等问题。     

网的收敛与分离公理

用Ｍｏｏｒｅ－Ｓｍｉｔｈ收敛给出了一个拓扑空间分别是Ｔ０，Ｔ１，正则，正规空间的充要条件．     

IS γα≤δ FOR GRAPHS WHICH HAVE DIAMETER TWO?

A subset of S of the vertex set of a graph G is called acyclic if the subgraph it induces in G contains no cycles. S is called an acyclic dominating set of G if it. is both acyclic and dominating. The minimum cardinality of an acyclic dominating set, denoted by 7a(G), is called the acyclic domination number of G. S. M. Hedetniemi et al. on 2000 introduced the concept of acyclic domination and posed the following open problem: Is -ya(G) < <5(G) for any graph whose diameter is two? In this paper, we give a counterexample which disproves the problem.     

路与路强乘积的最小直径定向

给定一个无向图G，将G的每条边{xy}．用弧xy或yx替代得到的有向图称为G的的定向图。使得G的所有定向图中直径最小的定向图称为G的最小直径定向。文章给出了两条路强乘积的最小直径定向。     

分数Hamilton圈与分数树形图

定义有向图的分数有向Hamilton圈和分数支撑树形图，讨论分数Hamilton圈、分数旅行售货员问题和分数支撑树形图基于线性规划的等价定义及多项式时间算法。