靶向重组Aif-T18融合蛋白对TEM1阳性肿瘤细胞的杀伤作用的研究

验证Aif-T18融合蛋白对肿瘤内皮细胞标志分子1(TEM1)阳性肿瘤细胞的杀伤作用。采用基因工程技术方法构建重组质粒pIRES-TEM1-EGFP,转染TEM1阴性表达细胞MS1,经G418筛选得到TEM1阳性细胞株(MS1-TEM1);通过构建重组质粒pET302-Aif和pET302-Aif-T18,转化至表达宿主菌BL21中,经异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导菌体得到所需的目的蛋白,采用流式细胞仪和MTT技术检测融合蛋白对MS1和MS1-TEM1细胞的亲和力和特异性杀伤的效果。本研究中,主要利用单链抗体sc FvT18选择性携带凋亡诱导因子AIF,靶向抑杀TEM1阳性细胞,从而为Aif-T18融合蛋白在肿瘤治疗中的开发应用提供实验依据。     

Cu基形状记忆合金中析出相的研究

本文利用 X 射线衍射分析、透射电镜等技术,研究了几种热处理制度下 Cu—27.84 Al—3.82 Ni(at%)合金中析出相;用会聚束(CBED)技术测定了析出相与马氏体的取向关系。结果表明:分级处理试样中析出相 r_2是复杂立方结构,晶格参数 a=0.87nm;大量弥散的折出相引起共格应变场,从而使分极处理试样 X 射线衍射峰型宽化;r_2与2H 马氏体的取向关系为;[00]2H‖[001]_r_2,(10)_(2H)‖(300)_r_2。     

TEM在内蒙古正镶白旗地区水文地质勘察中的应用

利用TEM法,对比分析不同测线视电阻率-深度断面图,得出地下岩层含水层的埋藏条件及富水性,初步确定取水井位;再结合区域水文地质条件,对比分析不同测线中单井涌水量的相对大小,最终确定理想井位.以TEM在内蒙古正镶白旗地区地下水勘察中的应用为例,证明了TEM在确定含水层及井位上的有效性.     

EUT对TEM传输室特性阻抗影响的边界元分析

采用边界元法分析了金属箱体的受试设备对横电磁波传输室特性阻抗的影响．计算结果表明，当受试设备不满足“三分之一准则”时，对特性阻抗的影响达5Ω以上，当满足“三分之一准则”时，对特性阻抗的影响小于2Ω．测量结果证实了计算方法的有效性，所得结论对TEM传输室的设计与测试具有重要意义。     

Therapeutical effect of Shengmaisan is determined by the interaction among the three herbs

Shengmaisan is a recipe of traditional Chinese medicine, which is composed of three herbs. The interaction among the components from the three herbs of Shengmaisan is investigated using FT-IR, Matrix Assistant Laser Desorption/Ionization Time of flight mass spectrometry (MALDI TOF MS) and TEM observation. Interactions among various components from different herbs cause enhancement of the solubility of some component and suppression of the solubility of others. Results from MALDI-TOF MS show that some components found in the decoction from a single herb disappear when different herbs are decocted together. On the other hand, new substances are produced when two and/or three herbs of Shengmaisan are decocted together. TEM showed that vacuoles formed in the decoction from two and/or three herbs of Shengmaisan but did not occur in any decoction from a single herb. This result provides us direct evidence that the solubilized structure is formed by the interaction among the components from different herbs. This knowledge helps us to understand the therapeutical effects of traditional Chinese medicine.     

Morphology of tube-like threads related to Limnochares aquatica (L., 1758) (Acariformes: Hydrachnidia: Limnocharidae) in the laboratory

Water mites Limnochares aquatica (L., 1758) during maintenance in the laboratory for a long period of time in constant conditions periodically produced certain whitish flocculent material consisting of long rigid unbranched tube-like threads 1.3 ± 0.3 µm in diameter crossing freely. These threads were studied using light-optical as well as transmission electron microscopical and scanning electron microscopical methods. Microbiological staining was also applied to the threads to exclude their bacterial or fungal origin. The thread wall is built of fine fibrils arranged at different angles to the long axis of threads that is reflected in a certain stratification of the wall. Threads are mostly hollow or may contain electron-dense homogeneous material. No cell components are present in the thread composition. Numerous dermal glands with their small slit-like orifice scattered throughout the mite body surface are thought to produce these threads. Most probably the thread formation is a reaction of mites to stress under laboratory conditions, and this is expected to be a type of defensive reaction.     

中国外语学习者英语读写能力发展现状——一项基于TEM8的研究

在我国,英语学习者的读写能力主要是通过课堂学习获得的.语言使用环境的缺失使得对EFL学习者的读写能力衡量只能通过测试来了解.TEM8是我国目前等级最高的英语测试,通过对TEM8阅读理解和写作部分成绩的调查,我们发现中国EFL学习者的英语读写能力亟需提高.     

制备特定尺寸的纳米金颗粒方法及性能表征

通过化学还原法制备出不同粒径的纳米金颗粒。利用紫外可见分光光度计和透射电子显微镜对纳米金颗粒的形貌及尺寸进行表征。讨论了还原剂种类、还原剂用量、试剂加入顺序、反应温度等因素对纳米金颗粒稳定性、粒径、形貌和分散性的影响。结果表明：Na3C6H5O7为还原剂制得纳米金颗粒粒径在15-20 nm之间,NaBH4为还原剂制得的...     

Effect of Si on austenite stabilization,martensite morphology,and magnetic properties in Fe-26%Ni-x%Si alloys

The effect of Si on the austenite stabilization, martensite morphology, and magnetic properties in Fe-26%Ni-x%Si (x=3.5, 5, and 6) alloys have been studied by means of transmission electron microscopy (TEM) and M?ssbauer spectroscopy techniques. TEM observations reveal that the martensite morphology is closely dependent on the Si content. The volume fraction changes of martensite and austenite phases, the hyperfine magnetic field, and isomer shift values have been determined by Mssbauer spectroscopy. The M?ssbauer study reveals that the hyperfine magnetic field, the isomer shift values and the volume fraction of martensite decrease with increasing Si content.