发酵液中Monacolin K的高效液相色谱分析方法研究

对高效液相色谱(HPLC)测定发酵液中Monacolin　K的含量进行了研究，找到了最佳流动相配比和流速等条件。通过测定其重视性、回收率等项指标，证明此方法对有效物质的检测具有准确、快速的特点。     

体检人群中乳腺增生症患者的危险因素调查

使用自制调查问卷对参加莆田学院附属医院体检的3809名女性体检者进行调查,找出与乳腺增生发生密切相关的危险因素。调查结果显示,乳腺增生症发病率为43.84%,乳腺增生症患者更关注疾病本身。多因素非条件Logistic分析显示影响其发生危险因素是年龄、职业、性格特点、母乳喂养、饮食方式和断乳行为。结果表明,提高妇女对乳腺增生的认识,提倡母乳喂养,科学断乳行为,健康饮食,劳逸结合,能预防乳腺增生症的发生。     

HMG＿（14）单克隆抗体杂交瘤株的建立

以北京鸭红细胞提取的ＨＭＧｓ（ＨＭＧ＿１，ＨＭＧ＿（２ａ），ＨＭＧ＿（２ｂ），ＨＭＧ＿（１４），ＨＭＧ＿（１７））免疫Ｂａｌｂ／ｃ小鼠，取其脏压细胞与Ｓｐ２／０（小鼠骨髓瘤细胞）融合，经选择培养，阳性筛选，克隆化以及冻存复苏，获得了三株稳定分泌抗ＨＭＧ＿（１４）的杂交瘤细胞株（１Ｃ＿（１２），１Ｏ＿３和２Ｆ＿２），经ＷｅｓｔｅｒｎＢｌｏｔ鉴定，与其它几种ＨＭＧ无反应，抗体为ＩｇＧ＿１亚型。腹水ＨＭＧ＿（１４）单克隆抗体经ＳｅｐｈａｄａｘＣＬ－４Ｂ亲和柱得到了纯化。     

非直接荧光免疫法鉴定HMG＿（14）非组蛋白质在果蝇唾腺染色体上的原位分布

以获得的抗ＨＭＧ＿（１４）ＭｃＡｂ为探针,运用间接荧光免疫技术,对果蝇（Ｄ,Ｖｉｖｉｌｉｓ南京）唾腺染色体上ＨＭＧ＿（１４）的分布及其ＨＭＧ＿（１４）与活性区“ｐｕｆｆ”的关系进行了初步观察。发现;（１）ＨＭＣ＿（１４）分布在唾腺染色体所有的横纹带上;（２）在“ｐｕｆｆ”区,ＨＭＧ＿（１４）有增加的趋势;（３）ＤＮａｓｅＩ处理可以使染色体上的ＨＭＧ＿（１４）丢失。实验结果进一步验证了ＨＭＧ＿（１４）是染色体上的一种结构性蛋白。     

人尿促性腺激素中卵泡刺激素的分离与纯化

通过磺基水杨酸沉淀、ＳＰ－Ｓｅｐｈａｄｅｘ柱层的Ｒ试剂处理等步骤，将人尿促性腺激素粗品提纯为精品。此方法可将ＨＭＧ中卵泡刺激素比活提到３０－４０ｉｕ／ｍｇ，活力，回收率较高，简单易行，适于大批量生产。     

The potential therapeutic role of statins in central nervous system autoimmune disorders

3-Hydroxy-3-methyglutaryl coenzyme A (HMG-CoA) reductase inhibitors, statins are widely used oral cholesterol-lowering drugs. Statins competitively inhibit HMG-CoA reductase, the enzyme that catalyzes conversion of HMG-CoA to L-mevalonate, a key intermediate in cholesterol synthesis. Certain metabolites of mevalonate are also involved in posttranslational modification of specific proteins involved in cell proliferation and differentiation. Thus, statins have important biologic effects that may be independent of their cholesterol-reducing properties. Recent studies indicate that statins have antiinflammatory and neuroprotective properties which may be beneficial in the treatment of multiple sclerosis as well as other central nervous system (CNS) neurodegenerative diseases. This article will outline current experimental evidence that may suggest potential clinical benefits for patients with CNS autoimmune disorders. Ultimately, clinical trials will have to determine the safety and efficacy of statins in this patient population.Received 17 April 2003; received after revision 21 May 2003; accepted 22 May 2003     

乳腺增生病的中医证候规范化研究

调查乳腺增生病中医辨证分型现状发展,探讨乳腺增生病的证候规范化研究,为临床采用中医药治疗乳腺增生病提供相对客观、规范的诊断及疗效评价标准并有效指导临床辨证用药寻找理论依据,为临床建立客观性、适用性和可操作性强的证候诊断标准奠定基础。     

Interactions between HMG proteins (HMG1/2 and HMG14/17) and human ε-globin gene promoter (ε-promoter)

High mobility group (HMG) proteins are abundant non-histone proteins in the nuclei of eukaryocytes. It has been shown that HMG proteins may play important roles in the structure and function of chromatin. In the present study, thebinding of HMG proteins (HMG1/2 and HMG14/17) to the human ε-globin gene promoter (ε-promo- ter, -177-+1 bp) has been examined by using both the in vitro nucleosome reconstitution and the electrophoresis mobility shift assay (EMSA). We found that HMG1/2 proteins could bind to the naked ε-promoter DNA, however, HMG14/17 could not. Using the in vitro nucleosome recons- titution, we revealed that HMG14/17 could bind to the mononucleosome reconstituted in vitro with ε-promoter,whi- le HMG1/2 could not. Those results indicate that the binding of HMG proteins to ε-promoter is dynamic as the nucleo- some assembling and disassembling. Wespeculated that this selective binding of HMG proteins to ε-promoter might playa critical role in the regulation of ε-globin gene expression.