The phosphoinositide PI(3,5)P2 mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells

Two-pore channel proteins (TPC) encode intracellular ion channels in both animals and plants. In mammalian cells, the two isoforms (TPC1 and TPC2) localize to the endo-lysosomal compartment, whereas the plant TPC1 protein is targeted to the membrane surrounding the large lytic vacuole. Although it is well established that plant TPC1 channels activate in a voltage- and calcium-dependent manner in vitro, there is still debate on their activation under physiological conditions. Likewise, the mode of animal TPC activation is heavily disputed between two camps favoring as activator either nicotinic acid adenine dinucleotide phosphate (NAADP) or the phosphoinositide PI(3,5)P₂. Here, we investigated TPC current responses to either of these second messengers by whole-vacuole patch-clamp experiments on isolated vacuoles of Arabidopsis thaliana. After expression in mesophyll protoplasts from Arabidopsis tpc1 knock-out plants, we detected the Arabidopsis TPC1-EGFP and human TPC2-EGFP fusion proteins at the membrane of the large central vacuole. Bath (cytosolic) application of either NAADP or PI(3,5)P₂ did not affect the voltage- and calcium-dependent characteristics of AtTPC1-EGFP. By contrast, PI(3,5)P₂ elicited large sodium currents in hTPC2-EGFP-containing vacuoles, while NAADP had no such effect. Analogous results were obtained when PI(3,5)P₂ was applied to hTPC2 expressed in baker’s yeast giant vacuoles. Our results underscore the fundamental differences in the mode of current activation and ion selectivity between animal and plant TPC proteins and corroborate the PI(3,5)P₂-mediated activation and Na⁺ selectivity of mammalian TPC2.     

非圆齿轮-连杆型函数发生器的机构综合

给出了非圆齿轮－连杆型函数发生器综合时必须遵循的机构学制约条件，并提出了确定连杆机构处于极限位置时的非圆齿轮角速比的基本公式。以具有等速急回特性的曲柄滑块机构型函数发生器的机构综合为例，研制了有实用价值的非圆齿轮－连杆型函数发生器机构综合CAD软件。     

针织绒线外观质量的模糊综合评判

在针织绒线外观质量评定中，规定要对其条干、手感、外观和色泽四项指标由主义人员进行手感目测评定。本文运用模糊集合理论，对评定中具有模糊性的指标进行分析并加以综合评判，从而减少了在绒线外观质量评定中的主观片面性，使得评定更加合理。     

Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens

The eye lens is composed of fibre cells, which develop from the epithelial cells on the anterior surface of the lens. Differentiation into a lens fibre cell is accompanied by changes in cell shape, the expression of crystallins and the degradation of cellular organelles. The loss of organelles is believed to ensure the transparency of the lens, but the molecular mechanism behind this process is not known. Here we show that DLAD ('DNase II-like acid DNase', also called DNase IIbeta) is expressed in human and murine lens cells, and that mice deficient in the DLAD gene are incapable of degrading DNA during lens cell differentiation--the undigested DNA accumulates in the fibre cells. The DLAD-/- mice develop cataracts of the nucleus lentis, and their response to light on electroretinograms is severely reduced. These results indicate that DLAD is responsible for the degradation of nuclear DNA during lens cell differentiation, and that if DNA is left undigested in the lens, it causes cataracts of the nucleus lentis, blocking the light path.     

小圈条最大容量时的气孔直径

本文通过分析计算，导出了小圈条最大容量时的气孔直径d0，有助于新机设计和老机改造时参考。     

Experimental extraction of an entangled photon pair from two identically decohered pairs

Entanglement is considered to be one of the most important resources in quantum information processing schemes, including teleportation, dense coding and entanglement-based quantum key distribution. Because entanglement cannot be generated by classical communication between distant parties, distribution of entangled particles between them is necessary. During the distribution process, entanglement between the particles is degraded by the decoherence and dissipation processes that result from unavoidable coupling with the environment. Entanglement distillation and concentration schemes are therefore needed to extract pairs with a higher degree of entanglement from these less-entangled pairs; this is accomplished using local operations and classical communication. Here we report an experimental demonstration of extraction of a polarization-entangled photon pair from two decohered photon pairs. Two polarization-entangled photon pairs are generated by spontaneous parametric down-conversion and then distributed through a channel that induces identical phase fluctuations to both pairs; this ensures that no entanglement is available as long as each pair is manipulated individually. Then, through collective local operations and classical communication we extract from the two decohered pairs a photon pair that is observed to be polarization-entangled.     

航天器的稳定自旋对双程多普勒追踪测量的影响

多普革追踪是航天器测量的主要手段之一，在通过对绕月轨道变化的观测来精确测定月球引力场球协函数高阶项的研究中，要求在数十秒采样间隔内得的多普勒频率漂移精度要达到１ＭＨｚ，然而，由于采用了自转稳定姿态控制模式，航天器的旋转会对多普勒测量产生影响，介绍了这种影响以及消除这种影响的方法。     

基于洛毕达极限法则的机构奇异位置运动分析

提出了运用洛毕达极限法则分析机构通过死点或切换点的角速度或速比的新方法，并具体应用于具有切换点的GRASHOF neutral机构和GALLOWAY机构的运动分析，计算分析和实验结果证明：这两种特殊的平面双曲槽机构的运动周期是原动曲柄旋转二整周，而并非通常的一整周，发现当CALLOWAY机构的相对杆长差取很小值时，机构能输出具有超长近似停歇期和反向自锁特性的间歇运动，具有实用价值。     

Non-canonical Wnt signals regulate cytoskeletal remodeling in osteoclasts

Osteoclasts are multinucleated cells responsible for bone resorption. Osteoclasts adhere to the bone surface through integrins and polarize to form actin rings, which are formed by the assembly of podosomes. The area contained within actin rings (also called sealing zones) has an acidic pH, which causes dissolution of bone minerals including hydroxyapatite and the degradation of matrix proteins including type I collagen by the protease cathepsin K. Osteoclasts resorb bone matrices while moving on bone surfaces. Osteoclasts change their cell shapes and exhibit three modes for bone resorption: motile resorbing mode for digging trenches, static resorbing mode for digging pits, and motile non-resorbing mode. Therefore, the actin cytoskeleton is actively remodeled in osteoclasts. Recent studies have revealed that many molecules, such as Rac, Cdc42, Rho, and small GTPase regulators and effectors, are involved in actin cytoskeletal remodeling during the formation of actin rings and resorption cavities on bone slices. In this review, we introduce how these molecules and non-canonical Wnt signaling regulate the bone-resorbing activity of osteoclasts.