我国建筑结构质量现状分析

结合我国当前建筑结构质量状况 ,分析了质量事故发生的时间和原因 ,指出了改善、控制和维修等措施     

政治经济学课程教学改革的思考

在师范院校政教系政治经济学既是政治理论课又是专业基础课。搞好政治经济学的教学,不仅对于学生学习好后续课程具有重要的基础性意义,而且对于培养学生的理论兴趣、科学精神和科学方法、创新精神和实践能力也具有十分重要的作用。因此,作为政治经济学课教师必须不断改革,在教学内容、方法、手段上创新,大胆探索,勇于实践,才能使政治经济学的教学更加适应新世纪人才培养的需要。     

云南城市化目标的设定与战略选择

随着第二步战略目标的基本实现，我国的社会经济发展已经进入到全面建设小康社会的崭新的阶段，这个阶段意味着我国城乡生产力布局和人口的空间分布都会发生有史以来规模最大、最为深刻的变迁。全面建设小康社会的奋斗目标具有丰富的内涵，涉及广泛而丰富的社会经济结构，而推动城市化进程则是其重要内容之一。云南是一个欠发达的农业大省，农业劳动多，农村人口比重大，如何推进城市化，必须根据云南的实际，制定正确的城市化目标和发展战略。     

催化剂、基底对ECR-CVD法制备碳纳米管的影响

采用电子回旋共振微波等离子体化学气相沉积技术(ECR CVD),CH4和H2为气源,分别以Fe3O4,Co纳米粒子及Fe(NO)3溶胶为催化剂在多孔硅基底上制备了碳纳米管;在Si(111)和石英基底上以Fe3O4纳米粒子为催化剂实现了碳纳米管的生长·使用扫描电子显微镜(SEM)、透射电子显微镜(TEM)对样品的形貌、尺寸及结构进行表征·讨论了催化剂和基底对碳纳米管形貌、密度和取向性的影响·结果表明:催化剂影响碳纳米管的成核密度和生长速度,基底通过影响催化剂的特性和分布均匀性,对碳纳米管的形貌和生长模式产生重要影响,以Fe3O4为催化剂在多孔硅上实现了碳纳米管的最优定向生长·     

一种基于加权证据合成的多传感器目标识别方法

针对多传感器数据融合目标识别问题，基于D-S证据理论，提出了加权证据合成的时空域目标识别算法。该方法充分利用了多传感器多周期的测量数据，并根据D-S合成规则要求参与合成的各证据具有相同权重的特点，充分考虑了提供证据的信源即各个传感器的可靠性。在合成中，引入证据权的概念，解决了不同权重的多传感器数据融合问题，在一定程度上改善了目标识别系统的性能。最后通过计算实例表明算法是有效的。     

The stoichiometry of peptide-heparan sulfate binding as a determinant of uptake efficiency of cell-penetrating peptides

Binding to negatively charged heparan sulfates (HS) at the cell surface is considered the first step in the internalization of cationic cell-penetrating peptides (CPPs). However, little is known about the relation of the characteristics of the HS-CPP interaction such as affinity, stoichiometry, and clustering with uptake. In this study, we investigated a collection of mutants of a cyclic CPP derived from human lactoferrin with respect to HS binding and uptake. The thermodynamic parameters of HS binding were determined by isothermal titration calorimetry, clustering of HS was investigated by dynamic light scattering, and cellular uptake by flow cytometry and confocal microscopy. Whereas mutations of non-arginine amino acids that are conserved across lactoferrins of different mammalia only had a minor effect on uptake efficiency, changes in the number of arginine residues influenced the uptake significantly. In general, introduction of arginine residues and cyclization improved the HS affinity and the ability to cluster HS. In particular, there was a strong negative correlation between stoichiometry and uptake, indicating that crosslinking of HS is the driving force for the uptake of arginine-rich CPPs. Using glycan microarrays presenting a collection of synthetic HS, we show that a minimal chain length of HS is required for peptide binding.     

Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase

Notch signalling is a key intercellular communication mechanism that is essential for cell specification and tissue patterning, and which coordinates critical steps of blood vessel growth. Although subtle alterations in Notch activity suffice to elicit profound differences in endothelial behaviour and blood vessel formation, little is known about the regulation and adaptation of endothelial Notch responses. Here we report that the NAD(+)-dependent deacetylase SIRT1 acts as an intrinsic negative modulator of Notch signalling in endothelial cells. We show that acetylation of the Notch1 intracellular domain (NICD) on conserved lysines controls the amplitude and duration of Notch responses by altering NICD protein turnover. SIRT1 associates with NICD and functions as a NICD deacetylase, which opposes the acetylation-induced NICD stabilization. Consequently, endothelial cells lacking SIRT1 activity are sensitized to Notch signalling, resulting in impaired growth, sprout elongation and enhanced Notch target gene expression in response to DLL4 stimulation, thereby promoting a non-sprouting, stalk-cell-like phenotype. In vivo, inactivation of Sirt1 in zebrafish and mice causes reduced vascular branching and density as a consequence of enhanced Notch signalling. Our findings identify reversible acetylation of the NICD as a molecular mechanism to adapt the dynamics of Notch signalling, and indicate that SIRT1 acts as rheostat to fine-tune endothelial Notch responses.     

Persistence of soil organic matter as an ecosystem property

Globally, soil organic matter (SOM) contains more than three times as much carbon as either the atmosphere or terrestrial vegetation. Yet it remains largely unknown why some SOM persists for millennia whereas other SOM decomposes readily--and this limits our ability to predict how soils will respond to climate change. Recent analytical and experimental advances have demonstrated that molecular structure alone does not control SOM stability: in fact, environmental and biological controls predominate. Here we propose ways to include this understanding in a new generation of experiments and soil carbon models, thereby improving predictions of the SOM response to global warming.     

NH3-TPD法表征固体催化剂的酸性

采用NH3-TPD法,利用FINESORB-3010程序升温化学吸附仪测定固体催化剂表面酸性.研究样品量、样品的预处理、炉温和程序升温速率、反应气和载气流速、桥电流设置以及气体选择等对测定固体催化剂酸性的影响.