Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance.

A cloned BamH1-generated fragment of ColV,I-K94 increased the virulence of Escherichia coli, causing an approximately 100-fold reduction in LD50 for chicks. A genetic determinant for resistance to the bactericidal effects of serum was mapped to a 5,300 base-pair sequence within the fragment. Neither colicin V nor immunity to colicin V affected the pathogenicity of E. coli for chicks.     

Cytotoxic potential of lymphocytes stimulated with autochthonous lymphoid cell line cells

Résumé Les petits lymphocytes du sang, après avoir été mis en contact pendant plusieurs jours avec les cellules lymphoïdes d'une lignée autochthone, peuvent tuer les cellules de cette lignée et d'autres lignées. Ces investigations peuvent apporter de nouvelles données sur l'opération de contrôle immunologique.

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We are grateful to Dr.N. R. Ling for his help and guidance.D. A. Hardy gratefully acknowledges financial support from the Medical Research Council and the John Squire Fund.     

Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region

The Ia antigens of the mouse are the basis for the genetic control of the immune response. The HLA-D/DR locus is considered to be the human counterpart of the Ia subregion of the murine major histocompatibility complex. The HLA-D/DR antigens are polymorphic, and eight well defined alleles have been identified using alloantisera. More recently, 'supertypic' antigens (MB and MT) have been defined which identify clusters of HLA-D/DR specificities. Little is known about the molecular basis for the cellular and serological polymorphism of the HLA-D/DR antigens, as alloantisera are usually of very low titre and heteroantisera frequently lack monospecificity. We present here the preparation and characterization of a monoclonal antibody which defines a new polymorphic system of the HLA-D/DR region. This and similar antisera should now begin to provide the reagents with which to correlate molecular structure with the functional repertoire of the human Ia-like antigens.