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Construction of Yeast Vectors with Resistance to Geneticin 总被引：2，自引：0，他引：2
IntroductionYeast Saccharomyces cerevisiae (S.cerevisiae) hasbeen widely used in molecular biology as well asinthe industrial production.Its genetic backgroundhas been well studied and a series of cloning andexpression vectors have been constructed for DNAtransformation into yeast[1 ] .The selection markersof these conventional vectors are usually H IS3 ,TRP1 ,LEU2 ,and URA3 [1 ,2 ] . These selectionmarkers are responsible for the biosynthesis ofsome amino acids and consequently the hos… 相似文献
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IntroductionPolyhydroxyalkanoates,abbreviated as PHA,is afamily of intracellular biopolymers synthesized bymany bacteria as carbon and energy storagecompounds[1,2 ] . Attempts have been made toproduce PHA for biodegradable packaging[3 5] .Various bacterial strains have different PHAbiosynthesis pathways. Gern et al,studying thebiosynthesis pathway of Pseudomonasputida using13C nuclear magnetic resonance,concluded that thestrain synthesized PHA via the de novo fatty acidsynthesis pathway … 相似文献
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