靶向重组Aif-T18融合蛋白对TEM1阳性肿瘤细胞的杀伤作用的研究

验证Aif-T18融合蛋白对肿瘤内皮细胞标志分子1(TEM1)阳性肿瘤细胞的杀伤作用。采用基因工程技术方法构建重组质粒pIRES-TEM1-EGFP,转染TEM1阴性表达细胞MS1,经G418筛选得到TEM1阳性细胞株(MS1-TEM1);通过构建重组质粒pET302-Aif和pET302-Aif-T18,转化至表达宿主菌BL21中,经异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导菌体得到所需的目的蛋白,采用流式细胞仪和MTT技术检测融合蛋白对MS1和MS1-TEM1细胞的亲和力和特异性杀伤的效果。本研究中,主要利用单链抗体sc FvT18选择性携带凋亡诱导因子AIF,靶向抑杀TEM1阳性细胞,从而为Aif-T18融合蛋白在肿瘤治疗中的开发应用提供实验依据。     

Die Wirkung des Toluidinblaus und der Thrombokinase auf den Vorgang der Thrombininaktivierung

Summary The disappearance of thrombin—formed in the blood, or added to serum-follows a manomolecular reaction-type. Heparin increases the reaction-velocity of this thrombin-inactivating process.Our investigation established that toluidine blue or kinase, which, according to the literature, bind heparin, strongly reduce the speed of thrombin-inactivation too. Therefore the heparin-binding capacity of these substances is also manifested in the decrease of thrombin-inactivation.     

Mutations in LMF1 cause combined lipase deficiency and severe hypertriglyceridemia

Hypertriglyceridemia is a hallmark of many disorders, including metabolic syndrome, diabetes, atherosclerosis and obesity. A well-known cause is the deficiency of lipoprotein lipase (LPL), a key enzyme in plasma triglyceride hydrolysis. Mice carrying the combined lipase deficiency (cld) mutation show severe hypertriglyceridemia owing to a decrease in the activity of LPL and a related enzyme, hepatic lipase (HL), caused by impaired maturation of nascent LPL and hepatic lipase polypeptides in the endoplasmic reticulum (ER). Here we identify the gene containing the cld mutation as Tmem112 and rename it Lmf1 (Lipase maturation factor 1). Lmf1 encodes a transmembrane protein with an evolutionarily conserved domain of unknown function that localizes to the ER. A human subject homozygous for a deleterious mutation in LMF1 also shows combined lipase deficiency with concomitant hypertriglyceridemia and associated disorders. Thus, through its profound effect on lipase activity, LMF1 emerges as an important candidate gene in hypertriglyceridemia.     

浅谈新型墙体材料——煤矸石空心砖

煤矸石空心砖是一种新型环保墙体材料，符合国家及山西省出台的限制生产和使用实心黏土砖，鼓励发展新型建筑材料的政策。利用煤矸石制造的空心砖可以作为保温材料，既可节约建筑能耗，又可节约土地资源，减少环境污染。煤矸石空心砖同黏土实心砖相比，具有高强、轻质、隔音、隔热、保温、防震等优点，市场前景良好。     

A Framework to Support Agility Strategy Formulation

In the last decade, turbulent times and uncertainti es in the business environment have made ground for a new business era, and “chang e“ has become a major characteristic of the new era. This has resulted in tirele ss evolution of business systems and the creation of new manufacturing and manag ement philosophies. Agile Manufacturing (AM) is a step forward in generation of new means for better performance and success of business, and in practice is a s trategic approach to manufacturing by considering ...     

金属基复合材料界面层阻尼功能研究

采用CVD技术制备了具有不同界面层的C-f/Al金属基复合材料，获得了一种界面层阻尼功能设计的新方法. 研究发现具有特殊界面层的C-f/Al复合材料的抗拉强度、弹性模量和阻尼性能比无界面层时都有明显增加，并且不同界面层的效果不同. 碳层对复合材料阻尼性能的提高效果最大，硅层的提高效果不如碳层，碳硅混合层的效果居中. 涂层的厚度也影响了阻尼提高的效果，较厚的碳层效果更好，这是由于提高了复合材料的阻尼应变振幅效应而产生的. 研究认为发生在界面层的微滑移是其主要的阻尼机制.     

"非典"时期湖南民众社会心理特征的调查研究

通过问卷调查，首先对湖南民众在非典时期的社会心理状况进行描述分析，随后对不同年龄、化程度、职业类型的民众的社会心理特征进行了比较研究，并在此基础上为战胜非典的舆论导向和领导决策提供了相应的心理学依据和对策。     

一类供应商逆向选择问题的报酬机制研究

研究了采购企业在缺乏供应商实际业务能力、服务成本等方面信息的情况下 ,利用博弈论显示原理设计不同的供货合同来让供应商选择 ,从而根据供应商的选择结果来判断其真实的能力或类型 ,从而避免了供应商逆向选择问题的发生 .通过分析 ,得出了最优报酬机制的特征 ,并证明了上述报酬机制下的最优合同与采购企业对供应商综合能力和成本的先验认识无关 .