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1.
MacArthur DG Seto JT Raftery JM Quinlan KG Huttley GA Hook JW Lemckert FA Kee AJ Edwards MR Berman Y Hardeman EC Gunning PW Easteal S Yang N North KN 《Nature genetics》2007,39(10):1261-1265
More than a billion humans worldwide are predicted to be completely deficient in the fast skeletal muscle fiber protein alpha-actinin-3 owing to homozygosity for a premature stop codon polymorphism, R577X, in the ACTN3 gene. The R577X polymorphism is associated with elite athlete status and human muscle performance, suggesting that alpha-actinin-3 deficiency influences the function of fast muscle fibers. Here we show that loss of alpha-actinin-3 expression in a knockout mouse model results in a shift in muscle metabolism toward the more efficient aerobic pathway and an increase in intrinsic endurance performance. In addition, we demonstrate that the genomic region surrounding the 577X null allele shows low levels of genetic variation and recombination in individuals of European and East Asian descent, consistent with strong, recent positive selection. We propose that the 577X allele has been positively selected in some human populations owing to its effect on skeletal muscle metabolism. 相似文献
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Starch-binding domains in the post-genome era 总被引:1,自引:1,他引:0
Starch belongs to the most abundant biopolymers on Earth. As a source of energy, starch is degraded by a large number of various
amylolytic enzymes. However, only about 10% of them are capable of binding and degrading raw starch. These enzymes usually
possess a distinct sequence-structural module, the so-called starchbinding domain (SBD). In general, all carbohydrate-binding
modules (CBMs) have been classified into the CBM families. In this sequence-based classification the individual types of SBDs
have been placed into seven CBM families: CBM20, CBM21, CBM25, CBM26, CBM34, CBM41 and CBM45. The family CBM20, known also
as a classical C-terminal SBD of microbial amylases, is the most thoroughly studied. The three-dimensional structures have
already been determined by X-ray crystallography or nuclear magnetic resonance for SBDs from five CBM families (20, 25, 26,
34 and 41), and the structure of the CBM21 has been modelled. Despite differences among the amino acid sequences, the fold
of a distorted β-barrel seems to be conserved together with a similar way of substrate binding (mainly stacking interactions
between aromatic residues and glucose rings). SBDs have recently been discovered in many non-amylolytic proteins. These may,
for example, have regulatory functions in starch metabolism in plants or glycogen metabolism in mammals. SBDs have also found
practical uses.
Received 25 May 2006; received after revision 26 June 2006; accepted 3 August 2006 相似文献
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Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photoreceptors responsible for red light perception in plants, fungi and bacteria. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states, thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses. Several structures of microbial phytochromes have been determined in their dark-adapted Pr or Pfr states. However, the structural nature of initial photochemical events has not been characterized by crystallography. Here we report the crystal structures of three intermediates in the photoreaction of Pseudomonas aeruginosa bacteriophytochrome (PaBphP). We used cryotrapping crystallography to capture intermediates, and followed structural changes by scanning the temperature at which the photoreaction proceeded. Light-induced conformational changes in PaBphP originate in ring D of the biliverdin (BV) chromophore, and E-to-Z isomerization about the C(15) = C(16) double bond between rings C and D is the initial photochemical event. As the chromophore relaxes, the twist of the C(15) methine bridge about its two dihedral angles is reversed. Structural changes extend further to rings B and A, and to the surrounding protein regions. These data indicate that absorption of a photon by the Pfr state of PaBphP converts a light signal into a structural signal via twisting and untwisting of the methine bridges in the linear tetrapyrrole within the confined protein cavity. 相似文献
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