极谱法测定植物中聚(ADP-核糖基)聚合酶活性

建立了一种简便，灵敏，可信的ＰＡＲＰ活性测定的非同位素方法，即用线性扫描极谱法测出植物中ＰＡＲＰ酶促反应产物烟酰胺的含量，从而测定了ＰＡＲＰ的活性。ＮＩＣ的检测限达０．０３μｍｏｌ／Ｌ，线性关系为０．０６－５μｍｏｌ／Ｌ，相关系数ｒ＝０．９９９，ＮＩＣ的回收率约达９２％－９８％，相对标准偏差小于６．６％《     

大王庄油田地面污水处理工艺技术应用

本文从立式除油工艺技术原理，多功能过滤技术工艺原理，污水处理药剂防垢、缓蚀、杀菌机理，污水污油回收及污泥排放工艺流程，大王庄油田污水处理工艺技术应用效果分析介绍了大王庄油田污水处理主要技术工艺，并对污水处理工艺存在问题与技术完善进行了分析和说明。     

Induction of Apoptosis in Purified Nuclei from Tobacco-Suspension Cells by Cytochrome b6／f Complex

An apoptotic cell-free system containing cytosol and nuclei from normally cultured tobacco suspension cells was used to show that a spinach chloroplast preparation can induce apoptosis in nuclei, evidenced by DNA electrophoresis and fluorescence microscopy observations, Further study showed that the chloroplast preparation or its pellet (thylakoid membrane) after hypoosmotic or supersonic treatment still exhibited the apoptosis-inducing activity, but the supernatant had no effect, which indicates that the apoptosisinducing effector in the chloroplast preparation is water-insoluble. The induction of apoptosis by chloroplast preparation could be attenuated by Ac-DEVD-CHO, the specific inhibitor of Caspase-3, implying involvement of a Caspase-3-1ike protease during the process. Furthermore, extensive apoptosis in nuclei was induced by cytochrome b6/f on the thylakoid membrane, indicating that this important cytochrome complex may have an important role in the chloroplast-related apoptotic pathway.     

大叶白麻叶化学成分的研究

从大叶白麻[poacynumhendersonii(Hookf)woodson]叶中分得2个木脂素类化舍物,经理化常数测定和光谱分析,分别鉴定为(-)-丁香脂素[(-)-Syringaresino1]和( )-松脂素-4-O-β-D-葡萄糖甙[( )-Pinoresionol-4-0-β-D-glucopyranoside].