Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals

Summary [³H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.Supported in part by United States Public Health Service grant CA-17658 from the National Cancer Institute, and grant RR-05648 from the National Institutes of Health.     

Biochemical modifications of the crystalline lens in the experimental hypercholesteremia of rabbits

Résumé Les auteurs ont examiné par des méthodes biochimiques, en particulier celle deKingsley etSchaffert, le cristallin des lapins souffrant d'hypercholestérolémie expérimentale. Comparés aux contrôles, les animaux traités, ont présenté une augmentation de cholestérol de 24,6%, cette différence est significative (p< 0,001).     

Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals.

[3H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.