Synthesis of Thermoresponsive Poly（ diethyleneglycol methacrylate-co-6-Ovinyladipoyl-D-glucopyranose） Glycopolymer via RAFT Polymerization

The monomer 6-O-vinyladipoyl-D-glucopyranose（ VAG）was synthesized by lipase catalyzed trans-esterification of divinyladipate with D-glucopyranose. A novel double hydrophilic glycopolymer poly（ diethyleneglycol methacrylate-co-6-Ovinyladipoyl-D-glucopyranose）（ P（ DEGMA-co-VAG）） with narrow polydispersity（ PDI） and thermosensitivity was prepared by reversible addition-fragmentation chain transfer（ RAFT）polymerization. P（ DEGMA-co-VAG） was characterized by1 H NMR,FTIR and gel permeation chromatography（ GPC）. The characterization of UV-visible spectroscopy showed that the micelles from glycopolymer P（ DEGMA-co-VAG） were thermo-responsive and the low critical solution temperature（ LCST） could be controlled by the molar ratio of monomers. When the molar ratio of DEGMA and VAG was 2∶ 1,the LCST of P（ DEGMA-co-VAG） was36 ℃ in aqueous solution,which could form nano micelles in the human body environment. It was found that P（ DEGMA-co-VAG）was non-toxic at 0. 1-1 mg / m L concentrations when incubated with pig iliac endothelial cells（ PIECs） for 24 h. Thus,the synthesized glycopolymers has great potential as drug delivery carriers.