Regional specialization of retinal glial cell membrane

Neural activity generates increases in extracellular K+ concentration, [K+]0, which must be regulated in order to maintain normal brain function. Glial cells are thought to play an important part in this regulation through the process of K+ spatial buffering: K+-mediated current flow through glial cells redistributes extracellular K+ following localized [K+]0 increases. As is the case in other glia, the retinal Müller cell is permeable almost exclusively to K+ . Recent experiments have suggested that this K+ conductance may not be distributed uniformly over the cell surface. In the present study, two novel techniques have been used to assess the Müller cell K+ conductance distribution. The results demonstrate that 94% of all membrane conductance lies in the endfoot process of the cell. This strikingly asymmetric distribution has important consequences for theories concerning K+ buffering and should help to explain the generation of the electroretinogram.     

The effects of secretin on urinary volume and electrolytes in normal subjects and patients with chronic pancreatic disease

Zusammenfassung Injektion von Sekretin ruft bei normalen Versuchspersonen Diuresis und Vermehrung der Natrium- und Alkali-Ausscheidung hervor. Diese Wirkungen sind vermindert bei Patienten mit chronischer Pankreaserkrankung. Der mögliche Mechanismus und die praktische Bedeutung dieser Beobachtungen werden diskutiert.

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Supported successively by grants from the Royal Free Hospital Endowment Fund.     

半胱氨酸亚金铵的合成、理化性质及电镀金应用

以半胱氨酸为配体合成一种新型亚金配合物NH4Au(Cys)2,对该配合物进行元素分析、红外光谱、紫外光谱、热失重分析和导电性测量等理化性质研究;以该亚金配合物为金源开展相关的电镀金工艺探索,并通过四因素三水平的正交试验获得其最佳条件参数;采用扫描电子显微镜(SEM)和X线衍射(XRD)对镀金层的表面质量进行探讨。研究结果表明:该目标产物的分子式为NH4Au(Cys)2·2H2O,该配合物中以半胱氨酸的巯基和金配位为成健特征,在170℃以下热稳定性较好,该亚金配合物是一个典型的离子化合物。在电流密度为200~300 A/m2,p H为10.5~12.0,温度为35~45℃,金质量浓度为15~25 g/L的电镀工艺条件下,得到粒度为0.5~1.0μm的单质金,且主要沿着(111)面进行生长。     

随机纳米碳管网络及其渗流性质

数值模拟了实验上构造纳米碳管网络的溶液沉积方法.与一般的随机网络模型不同,将碳管的长度计算在内,而且考虑了不同的空间相交位形.数值模拟发现网络的度分布为高斯分布,平均集聚系数约为0.11.当网络中碳管平均面密度取值在σ0=179 200根/cm2附近时,网络系综达到渗流.在临界点附近,网络的连通概率p、两极之间电导G、...     

Voltage-dependent calcium and potassium channels in retinal glial cells

Glial cells, which outnumber neurones in the central nervous system, have traditionally been considered to be electrically inexcitable and to play only a passive role in the electrical activity of the brain. Recent reports have demonstrated, however, that certain glial cells, when maintained in primary culture, possess voltage-dependent ion channels. It remains to be demonstrated whether these channels are also present in glial cells in vivo. I show here that Müller cells, the principal glial cells of the vertebrate retina, can generate 'Ca2+ spikes' in freshly excised slices of retinal tissue. In addition, voltage-clamp studies of enzymatically dissociated Müller cells demonstrate the presence of four types of voltage-dependent ion channels: a Ca2+ channel, a Ca2+-activated K+ channel, a fast-inactivating (type A) K+ channel and an inward-rectifying K+ channel. Currents generated by these voltage-dependent channels may enhance the ability of Müller cells to regulate extracellular K+ levels in the retina and may be involved in the generation of the electroretinogram.     

十六位微机教学实验系统分析与改进

本文在分析CCT－88／51／98教学实验系统监控程序的基础上，对该教学实验系统监控程序中存在的问题作出了一些改进。     

Single-copy insertion of transgenes in Caenorhabditis elegans

At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.