Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite

From the standpoints of both basic research and biotechnology, there is considerable interest in reaching a clearer understanding of the diversity of biological mechanisms employed during lignocellulose degradation. Globally, termites are an extremely successful group of wood-degrading organisms and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. Only recently have data supported any direct role for the symbiotic bacteria in the gut of the termite in cellulose and xylan hydrolysis. Here we use a metagenomic analysis of the bacterial community resident in the hindgut paunch of a wood-feeding 'higher' Nasutitermes species (which do not contain cellulose-fermenting protozoa) to show the presence of a large, diverse set of bacterial genes for cellulose and xylan hydrolysis. Many of these genes were expressed in vivo or had cellulase activity in vitro, and further analyses implicate spirochete and fibrobacter species in gut lignocellulose degradation. New insights into other important symbiotic functions including H2 metabolism, CO2-reductive acetogenesis and N2 fixation are also provided by this first system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation. Our results underscore how complex even a 1-microl environment can be.     

The inner jet of an active galactic nucleus as revealed by a radio-to-gamma-ray outburst

Blazars are the most extreme active galactic nuclei. They possess oppositely directed plasma jets emanating at near light speeds from accreting supermassive black holes. According to theoretical models, such jets are propelled by magnetic fields twisted by differential rotation of the black hole's accretion disk or inertial-frame-dragging ergosphere. The flow velocity increases outward along the jet in an acceleration and collimation zone containing a coiled magnetic field. Detailed observations of outbursts of electromagnetic radiation, for which blazars are famous, can potentially probe the zone. It has hitherto not been possible to either specify the location of the outbursts or verify the general picture of jet formation. Here we report sequences of high-resolution radio images and optical polarization measurements of the blazar BL Lacertae. The data reveal a bright feature in the jet that causes a double flare of radiation from optical frequencies to TeV gamma-ray energies, as well as a delayed outburst at radio wavelengths. We conclude that the event starts in a region with a helical magnetic field that we identify with the acceleration and collimation zone predicted by the theories. The feature brightens again when it crosses a standing shock wave corresponding to the bright 'core' seen on the images.     

Active galactic nuclei as scaled-up Galactic black holes

A long-standing question is whether active galactic nuclei (AGN) vary like Galactic black hole systems when appropriately scaled up by mass. If so, we can then determine how AGN should behave on cosmological timescales by studying the brighter and much faster varying Galactic systems. As X-ray emission is produced very close to the black holes, it provides one of the best diagnostics of their behaviour. A characteristic timescale--which potentially could tell us about the mass of the black hole--is found in the X-ray variations from both AGN and Galactic black holes, but whether it is physically meaningful to compare the two has been questioned. Here we report that, after correcting for variations in the accretion rate, the timescales can be physically linked, revealing that the accretion process is exactly the same for small and large black holes. Strong support for this linkage comes, perhaps surprisingly, from the permitted optical emission lines in AGN whose widths (in both broad-line AGN and narrow-emission-line Seyfert 1 galaxies) correlate strongly with the characteristic X-ray timescale, exactly as expected from the AGN black hole masses and accretion rates. So AGN really are just scaled-up Galactic black holes.