Purification and Inhibition Fungal Growthof Chitinases from Vibrio pacini

0　IntroductionChitinases (EC3.2 .1.14 )areenzymesthathydrolyzechitin ,ahomopolymerofβ 1,4linkedN acetylglucosamine .Thecompletedegradationofchitinchainsisusuallyachievedbyacom plexofenzymesactingtobreaktheinitialchainsintochitooligosac charides .Theseparateenzymesthenacttobreaktheseoligomersdownintotheircomponentmonomers.Thechitinolyticsystemex istsinawiderangeoforganisms,includingbacteria[1 ] ,fungi,higherplants[2 ] ,insects[3] ,crustaceans[4] andsomevertebrates,andplaysvariousrolesbothin…     

The determination of atmospheric phosphine in Ny-lesund

Phoshpine (PH3), a volatile and toxic gas, has been proved to commonly exist in the environment.The determination of gaseous phosphine and its emission fluxes in Ny-lesund is reported in this paper.Results showed that gaseous phosphine exist in Arctic atmosphere commonly and the concentration of phosphine ranged from 16.3-600.2 ng/m3, with the maximum concentration of phosphine in tundra areas and the minimum in surface air of ocean.The concentration of phosphine ranged from 65.79-1259 ng/m3 in colliery at...     

波浪计算中的一个局部变分法

本文提出了一个改进的局部变分法以计算波浪绕流问题，和已有的局部变分法相比，不增加新的未知量且易于编制计算机程序．     

海上交通仿真综述

海上交通仿真是海上智能交通运输系统（MITS）的一项重要内容,介绍了海上交通仿真的研究意义和研究内容,描述了国内外海上交通仿真的发展历程和研究现状,凝炼出海上交通仿真的关键技术。对基于仿真的海上交通领域的研究煞点问题,相关理论问题、方法和主要应用领域进行了探讨。最后指出了当前海上交通仿真研究的趋势。     

实时自适应波束形成器的神经网络方法

作者在Ｈｏｐｆｉｅｌｄ神经网的基础上构造了一种实时处理的自适应波束形成网络。由于实际应用中神经元的联结矩阵有时是奇异的，文章引入正则化方法，有效地提高了网络解的稳定性和处理速度。为了降低编程复杂性，避免计算求协方差矩阵，作者还改进了一种网络，该网络用模拟电路实现不需要任何计算，并且便于用常规的模拟电路实时处理。仿真实验结果表明，这种网比传统的自适应方法有较好的收敛和跟踪性能。     

Study on the photosynthetic performances of Enteromorpha prolifera collected from the surface and bottom of the sea of Qingdao sea area

In this work, the photosynthetic performances of Enteromorpha prolifera thalli collected from the sur- face and bottom of the sea of Qingdao sea area were studied with chlorophyll fluorescence and oxy- graph technology. The samples with the highest photosynthetic activity among their kinds, the floating thalli from the sea surface of the south of the Qingdao Olympic Sailing Center and the sedimentary thalli from the mud surface of the bottom Tuandao bay, were chosen as representatives of surface thalli and ...     

Inactivation of the p53 pathway in retinoblastoma

Most human tumours have genetic mutations in their Rb and p53 pathways, but retinoblastoma is thought to be an exception. Studies suggest that retinoblastomas, which initiate with mutations in the gene retinoblastoma 1 (RB1), bypass the p53 pathway because they arise from intrinsically death-resistant cells during retinal development. In contrast to this prevailing theory, here we show that the tumour surveillance pathway mediated by Arf, MDM2, MDMX and p53 is activated after loss of RB1 during retinogenesis. RB1-deficient retinoblasts undergo p53-mediated apoptosis and exit the cell cycle. Subsequently, amplification of the MDMX gene and increased expression of MDMX protein are strongly selected for during tumour progression as a mechanism to suppress the p53 response in RB1-deficient retinal cells. Our data provide evidence that the p53 pathway is inactivated in retinoblastoma and that this cancer does not originate from intrinsically death-resistant cells as previously thought. In addition, they support the idea that MDMX is a specific chemotherapeutic target for treating retinoblastoma.