建设计算机实验教学中心培养应用型人才

根据计算机应用型人才培养的特点和实践教学的要求,整合现有的实践教学资源,按照分层次、重特色建设思路,以持续不断的实验教学改革为动力,建成了四川省实验教学示范中心.文章总结了近几年实验教学示范中心建设的实践探索经验,包括开放平台建设、特色实验室建设、实验教学体系及实验教学内容建设、实验室开放内涵深化等.     

Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein

The Rho-family GTPase, Cdc42, can regulate the actin cytoskeleton through activation of Wiskott-Aldrich syndrome protein (WASP) family members. Activation relieves an autoinhibitory contact between the GTPase-binding domain and the carboxy-terminal region of WASP proteins. Here we report the autoinhibited structure of the GTPase-binding domain of WASP, which can be induced by the C-terminal region or by organic co-solvents. In the autoinhibited complex, intramolecular interactions with the GTPase-binding domain occlude residues of the C terminus that regulate the Arp2/3 actin-nucleating complex. Binding of Cdc42 to the GTPase-binding domain causes a dramatic conformational change, resulting in disruption of the hydrophobic core and release of the C terminus, enabling its interaction with the actin regulatory machinery. These data show that 'intrinsically unstructured' peptides such as the GTPase-binding domain of WASP can be induced into distinct structural and functional states depending on context.