长江水质评价和预测的模型研究

本文研究长江水质的评价与预测的模型。对长江近两年水质总体情况和地区分布进行了定性和定量分析。根据近10年(水文年)的主要统计数据进行分析,给出了I—V类、劣V类水无量纲百分比发展趋势和污水排放量发展趋势的超越函数混合模型和趋势图,由此预测未来长江水污染的情势。最后对解决长江水质污染问题提出了几点建议。     

Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage

The Cdc25A phosphatase is essential for cell-cycle progression because of its function in dephosphorylating cyclin-dependent kinases. In response to DNA damage or stalled replication, the ATM and ATR protein kinases activate the checkpoint kinases Chk1 and Chk2, which leads to hyperphosphorylation of Cdc25A. These events stimulate the ubiquitin-mediated proteolysis of Cdc25A and contribute to delaying cell-cycle progression, thereby preventing genomic instability. Here we report that beta-TrCP is the F-box protein that targets phosphorylated Cdc25A for degradation by the Skp1/Cul1/F-box protein complex. Downregulation of beta-TrCP1 and beta-TrCP2 expression by short interfering RNAs causes an accumulation of Cdc25A in cells progressing through S phase and prevents the degradation of Cdc25A induced by ionizing radiation, indicating that beta-TrCP may function in the intra-S-phase checkpoint. Consistent with this hypothesis, suppression of beta-TrCP expression results in radioresistant DNA synthesis in response to DNA damage--a phenotype indicative of a defect in the intra-S-phase checkpoint that is associated with an inability to regulate Cdc25A properly. Our results show that beta-TrCP has a crucial role in mediating the response to DNA damage through Cdc25A degradation.     

冷链运输装备开门渗风试验及温度场分析

冷藏运输与配送过程中开、关门进行装卸作业易造成货物温度波动,从而影响食品质量,亟待规范.研究对30℃/-18℃条件下冷链配送过程中存在问题进行分析,对装卸时间、是否安装门帘、装卸时是否开启循环风机等状况进行对比试验.在此基础上有针对性地提出完善及规范化的操作方案.认为开门时间长短是影响运输装备内部温度最重要的因素之一,在装卸作业时对大门开启处进行适当的保护则能大大降低冷量的损失,此外,装卸作业时车内循环风机以关闭为宜.     

构建农村经济安全体系的思考

近年来农村经济的发展出现了减缓趋势,农村面临的经济安全问题,必须引起我们高度重视。全面建设小康社会重点在农村,关键是发展农村经济。为了保证农村经济安全、健康、持续的发展,必须构建农村经济安全保障体系。     

论和谐社会构建中体育之促进作用

采用文献资料、逻辑推理、理论等方法,分析了和谐社会的构建拓宽了体育的功能范围,并对体育在和谐社会构建中的作用进行了论述,旨在更新观念,树立“和谐”意识。     

Control of the SCF(Skp2-Cks1) ubiquitin ligase by the APC/C(Cdh1) ubiquitin ligase

Skp2 and its cofactor Cks1 are the substrate-targeting subunits of the SCF(Skp2-Cks1) (Skp1/Cul1/F-box protein) ubiquitin ligase complex that regulates entry into S phase by inducing the degradation of the cyclin-dependent kinase inhibitors p21 and p27 (ref. 1). Skp2 is an oncoprotein that often shows increased expression in human cancers; however, the mechanism that regulates its cellular abundance is not well understood. Here we show that both Skp2 and Cks1 proteins are unstable in G1 and that their degradation is mediated by the ubiquitin ligase APC/C(Cdh1) (anaphase-promoting complex/cyclosome and its activator Cdh1). Silencing of Cdh1 by RNA interference in G1 cells stabilizes Skp2 and Cks1, with a consequent increase in p21 and p27 proteolysis. Depletion of Cdh1 also increases the percentage of cells in S phase, whereas concomitant downregulation of Skp2 reverses this effect, showing that Skp2 is an essential target of APC/C(Cdh1). Expression of a stable Skp2 mutant that cannot bind APC/C(Cdh1) induces premature entry into S phase. Thus, the induction of Skp2 and Cks1 degradation in G1 represents a principal mechanism by which APC/C(Cdh1) prevents the unscheduled degradation of SCF(Skp2-Cks1) substrates and maintains the G1 state.