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Role of the G segment in the growth of phage Mu 总被引:5,自引:0,他引:5
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A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum 总被引:19,自引:0,他引:19
Magalhaes JV Liu J Guimarães CT Lana UG Alves VM Wang YH Schaffert RE Hoekenga OA Piñeros MA Shaff JE Klein PE Carneiro NP Coelho CM Trick HN Kochian LV 《Nature genetics》2007,39(9):1156-1161
Crop yields are significantly reduced by aluminum toxicity on highly acidic soils, which comprise up to 50% of the world's arable land. Candidate aluminum tolerance proteins include organic acid efflux transporters, with the organic acids forming non-toxic complexes with rhizosphere aluminum. In this study, we used positional cloning to identify the gene encoding a member of the multidrug and toxic compound extrusion (MATE) family, an aluminum-activated citrate transporter, as responsible for the major sorghum (Sorghum bicolor) aluminum tolerance locus, Alt(SB). Polymorphisms in regulatory regions of Alt(SB) are likely to contribute to large allelic effects, acting to increase Alt(SB) expression in the root apex of tolerant genotypes. Furthermore, aluminum-inducible Alt(SB) expression is associated with induction of aluminum tolerance via enhanced root citrate exudation. These findings will allow us to identify superior Alt(SB) haplotypes that can be incorporated via molecular breeding and biotechnology into acid soil breeding programs, thus helping to increase crop yields in developing countries where acidic soils predominate. 相似文献
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A Hugelin T Tarrade M Istin R Coelho 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1977,285(15):1339-1341
16 hrs. after per os administration of 14C N-isopropyl-amino-2-pyrimidine (IAP) in the Rat, radioactivity of the sciatic nerve is significantly higher than in plasma and other organs. In vitro IAP (base, orthophosphate or dichloracetate) accelerates neurite outgrowth of explained spinal ganglion after 48 hrs. of incubation in nutritive medium. Results suggest that IAP incorporated in nerve cell acts on cellular mechanisms controlling nerve growth. 相似文献
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Large-scale analysis of the yeast genome by transposon tagging and gene disruption 总被引:67,自引:0,他引:67
Ross-Macdonald P Coelho PS Roemer T Agarwal S Kumar A Jansen R Cheung KH Sheehan A Symoniatis D Umansky L Heidtman M Nelson FK Iwasaki H Hager K Gerstein M Miller P Roeder GS Snyder M 《Nature》1999,402(6760):413-418
Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken. 相似文献
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