排序方式: 共有23条查询结果,搜索用时 34 毫秒
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Voineagu I Wang X Johnston P Lowe JK Tian Y Horvath S Mill J Cantor RM Blencowe BJ Geschwind DH 《Nature》2011,474(7351):380-384
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Noise in eukaryotic gene expression 总被引:56,自引:0,他引:56
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Construction of a genetic toggle switch in Escherichia coli 总被引:28,自引:0,他引:28
It has been proposed' that gene-regulatory circuits with virtually any desired property can be constructed from networks of simple regulatory elements. These properties, which include multistability and oscillations, have been found in specialized gene circuits such as the bacteriophage lambda switch and the Cyanobacteria circadian oscillator. However, these behaviours have not been demonstrated in networks of non-specialized regulatory components. Here we present the construction of a genetic toggle switch-a synthetic, bistable gene-regulatory network-in Escherichia coli and provide a simple theory that predicts the conditions necessary for bistability. The toggle is constructed from any two repressible promoters arranged in a mutually inhibitory network. It is flipped between stable states using transient chemical or thermal induction and exhibits a nearly ideal switching threshold. As a practical device, the toggle switch forms a synthetic, addressable cellular memory unit and has implications for biotechnology, biocomputing and gene therapy. 相似文献
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Retinoic acid was administered to hamsters suffering from N-nitroso-N-methylurethane-induced fibrosing alveolitis. A significant increase in macrophage numbers was seen in the lungs of retinoid-treated animals as compared to the unsupplemented group. 相似文献
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Guido NJ Wang X Adalsteinsson D McMillen D Hasty J Cantor CR Elston TC Collins JJ 《Nature》2006,439(7078):856-860
The ability to construct synthetic gene networks enables experimental investigations of deliberately simplified systems that can be compared to qualitative and quantitative models. If simple, well-characterized modules can be coupled together into more complex networks with behaviour that can be predicted from that of the individual components, we may begin to build an understanding of cellular regulatory processes from the 'bottom up'. Here we have engineered a promoter to allow simultaneous repression and activation of gene expression in Escherichia coli. We studied its behaviour in synthetic gene networks under increasingly complex conditions: unregulated, repressed, activated, and simultaneously repressed and activated. We develop a stochastic model that quantitatively captures the means and distributions of the expression from the engineered promoter of this modular system, and show that the model can be extended and used to accurately predict the in vivo behaviour of the network when it is expanded to include positive feedback. The model also reveals the counterintuitive prediction that noise in protein expression levels can increase upon arrest of cell growth and division, which we confirm experimentally. This work shows that the properties of regulatory subsystems can be used to predict the behaviour of larger, more complex regulatory networks, and that this bottom-up approach can provide insights into gene regulation. 相似文献
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J. O. Cantor S. S. Shapiro P. A. di Sant'Agnese J. M. Cerreta P. W. Trown 《Cellular and molecular life sciences : CMLS》1979,35(7):895-896
Summary Retinoic acid was administered to hamsters suffering from N-nitroso-N-methylurethane-induced fibrosing alveolitis. A significant increase in macrophage numbers was seen in the lungs of retinoid-treated animals as compared to the unsupplemented group. 相似文献
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Physical and enzymatic studies on poly d(I-C)-poly d(I-C), an unusual double-helical DNA 总被引:18,自引:0,他引:18
Y Mitsui R Langridge B E Shortle C R Cantor R C Grant M Kodama R D Wells 《Nature》1970,228(5277):1166-1169