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1.
Patricio Atanes Inmaculada Ruz-Maldonado Ross Hawkes Bo Liu Min Zhao Guo Cai Huang Israa Mohammed Al-Amily Albert Salehi Stefan Amisten Shanta J. Persaud 《Cellular and molecular life sciences : CMLS》2018,75(16):3039-3050
Introduction
Islets synthesise and secrete numerous peptides, some of which are known to be important regulators of islet function and glucose homeostasis. In this study, we quantified mRNAs encoding all peptide ligands of islet G protein-coupled receptors (GPCRs) in isolated human and mouse islets and carried out in vitro islet hormone secretion studies to provide functional confirmation for the species-specific role of peptide YY (PYY) in mouse islets.Materials and methods
GPCR peptide ligand mRNAs in human and mouse islets were quantified by quantitative real-time PCR relative to the reference genes ACTB, GAPDH, PPIA, TBP and TFRC. The pathways connecting GPCR peptide ligands with their receptors were identified by manual searches in the PubMed, IUPHAR and Ingenuity databases. Distribution of PYY protein in mouse and human islets was determined by immunohistochemistry. Insulin, glucagon and somatostatin secretion from islets was measured by radioimmunoassay.Results
We have quantified GPCR peptide ligand mRNA expression in human and mouse islets and created specific signalomes mapping the pathways by which islet peptide ligands regulate human and mouse GPCR signalling. We also identified species-specific islet expression of several GPCR ligands. In particular, PYY mRNA levels were ~ 40,000-fold higher in mouse than human islets, suggesting a more important role of locally secreted Pyy in mouse islets. This was confirmed by IHC and functional experiments measuring insulin, glucagon and somatostatin secretion.Discussion
The detailed human and mouse islet GPCR peptide ligand atlases will allow accurate translation of mouse islet functional studies for the identification of GPCR/peptide signalling pathways relevant for human physiology, which may lead to novel treatment modalities of diabetes and metabolic disease.2.
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采用文献资料法,依次从学理脉络、统计内容及其实施策略展开关于体育消费以及体育消费统计的讨论。认为:从学理上看,体育消费作为一个复杂概念,既牵涉宏观经济社会发展,也与文化意识形态紧密联系;而作为准确把握体育市场、把脉体育发展情况的基础性工作之一,体育消费统计则应该避免将体育消费概念泛化。应以体育政策为导向,面向人民的美好生活需要,对接国民经济统计制度,考虑建构以“体育教育与培训、体育文化与娱乐、体育保健与康复、体育传媒与信息、体育衣着耐用品、其他体育用品与服务、个人间接体育消费”为核心指标的统计框架,并通过长效工作方案的设计使体育消费统计工作制度化,通过统计调查制度的建构使体育消费统计执行有效化,通过统计数据的测算推算使体育消费统计结果精准化。 相似文献
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Archive for History of Exact Sciences - We show that Dedekind, in his proof of the principle of definition by mathematical recursion, used implicitly both the concept of an inductive cone from an... 相似文献
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Danielle Kamato Muhamad Ashraf Rostam Rebekah Bernard Terrence J. Piva Nitin Mantri Daniel Guidone Wenhua Zheng Narin Osman Peter J. Little 《Cellular and molecular life sciences : CMLS》2015,72(4):799-808
G protein-coupled receptor (GPCR) signalling is mediated through transactivation-independent signalling pathways or the transactivation of protein tyrosine kinase receptors and the recently reported activation of the serine/threonine kinase receptors, most notably the transforming growth factor-β receptor family. Since the original observation of GPCR transactivation of protein tyrosine kinase receptors, there has been considerable work on the mechanism of transactivation and several pathways are prominent. These pathways include the “triple membrane bypass” pathway and the generation of reactive oxygen species. The recent recognition of GPCR transactivation of serine/threonine kinase receptors enormously broadens the GPCR signalling paradigm. It may be predicted that the transactivation of serine/threonine kinase receptors would have mechanistic similarities with transactivation of tyrosine kinase pathways; however, initial studies suggest that these two transactivation pathways are mechanistically distinct. Important questions are the relative importance of tyrosine and serine/threonine transactivation pathways, the contribution of transactivation to overall GPCR signalling, mechanisms of transactivation and the range of cell types in which this phenomenon occurs. The ultimate significance of transactivation-dependent signalling remains to be defined but it appears to be prominent and if so will represent a new cell signalling frontier. 相似文献
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赵普春 《西安石油大学学报(自然科学版)》1996,(4)
针对文明寨油田地质特征和开采现状,按照油藏数值模拟原理,以调剖井对应油井的增油降水创经济效益大小为考核指标,运用正交试验的均衡分散性和整齐可比性及L9(34),正交表进行调剖最佳施工参数试验.经现场试验、分析、讨论发现:影响调剖效果的主要因素是措施半径,其次为施工排量和注入方式,且最佳施工参数是措施半径在3.5m左右,施工排量在19m3/h左右,按连续注入方式调剖。经文明察油田、卫城油田、马寨油田50井次现场试验证明,较好地改善了注水井吸水剖面,对应油井取得了明显的增油降水效果,收到了较好的经济和社会效益. 相似文献
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水轮机水力稳定性的分析 总被引:2,自引:0,他引:2
根据收集国内外水轮机水力稳定性资料 ,分析了引起水轮机水力不稳定性的因素 ,并结合实例分析可能引起的危害 ,最后提出了解决问题的方法和建议 相似文献