Bacterial utilization of cyclo(glycl-L-prolyl).

3 strains of soil bacteria (2 Achromobacter, 1 Flavobacterium) were isolated by growth on cyclo(Gly-L-Pro) as carbon/nitrogen source. Good growth required yeast extract supplements. Utilization of cyclo(Gly-L-Pro) was inducible. Many efforts failed to obtain active cell-free preparations. Injected radioactive cyclo(Gly-L-Pro) was excreted intact by the albino rat; in contrast, injected radioactive H-Gly-L-Pro-OH was extensively metabolized.     

Bacterial utilization of cyclo(glycyl-L-prolyl)

Summary 3 strains of soil bacteria (2Achromobacter, 1Flavobacterium) were isolated by growth on cyclo(Gly-L-Pro) as carbon/nitrogen source. Good growth required yeast extract supplements. Utilization of cyclo(Gly-L-Pro) was inducible. Many efforts failed to obtain active cell-free preparations. Injected radioactive cyclo(Gly-L-Pro) was excreted intact by the albino rat; in contrast, injected radioactive H-Gly-L-Pro-OH was extensively metabolized.Supported by Grant GM-11105 from the National Institutes of Health.     

Stable germline transformation of the malaria mosquito Anopheles stephensi

Anopheline mosquito species are obligatory vectors for human malaria, an infectious disease that affects hundreds of millions of people living in tropical and subtropical countries. The lack of a suitable gene transfer technology for these mosquitoes has hampered the molecular genetic analysis of their physiology, including the molecular interactions between the vector and the malaria parasite. Here we show that a transposon, based on the Minos element and bearing exogenous DNA, can integrate efficiently and stably into the germ line of the human malaria vector Anopheles stephensi, through a transposase-mediated process.