Mineralized Composite Nanofibrous Mats for Bone Tissue Engineering

Composite nanofibrous mats consisting of poly( L-lactideco-ε-caprolactone)( PLCL) and collagen type I( COL) were fabricated by electrospinning,and ten times simulated body fluid(10SBF) were employed to mineralize nanofibrous mats. Ballshaped hydroxyapatite( HA) was deposited on the surface of nanofibrous mats in 1. 5 h at room temperature. Human fetal osteoblasts( hFob) were seeded to investigate their proliferation and differentiation on mineralized composite nanofibrous mats. The results showed that hFob grew well on mineralized composite nanofibrous mats and alkaline phosphatase( ALP) activity of hFob on mineralized composite nanofibrous mats at 14 d was much higher than that on untreated nanofibrous mats. Moreover,the expression of osteocalcin of cells on mineralized composite nanofibrous mats was also much higher than those on untreated nanofibrous mats at 7 d and 14 d. This mineralized composite nanofibrous mats may have a great potential for bone tissue engineering.