苏云金杆菌H3-111菌株对刺蛾的毒效

苏云金杆菌H3-111菌株摇瓶培养液，室内测定48h对刺蛾幼虫毒力回归方程为Y=4.2900 1.9898x,y=0.9452，与标准品比较其生物效价为3300.621U.mg^-1；大田测试表明BtH3-111菌株防治刺蛾高峰期在药后72h-96h，稀释500-1000倍的防效达85.71%-89.10%，略高于DDVP。     

Microbiological Characteristics of Anaerobic Granular Sludge in Hybrid Anaerobic Baffled Reactor

Anaerobic granular sludge is of key importance for highly effective operation of hybrid anaerobic baffled reactor (HABR).An observation and analysis on the composition of anaerobic granular sludge in each separation compartment of HABR was conducted by using scanning electron microscope (SEM)and molecular biotechnology,and specific methanogenic activity(SMA) and coenzyme F420 content were determined. It was indicated that the disparity of microbial composition was significant among these separation compartments of HABR,and the HABR encouraged phase separation.The results show the understanding of microbiological characteristics of anaerobic granular sludge in HABR is helpful for cultivating granular sludge, which ensures the effective operation of the reactor.     

库拉索芦荟茎尖培养技术研究

切取生长健壮的库拉索芦荟茎尖，在无菌条件下，接种至含有不同植物生长调节剂种类和浓度，在不同温度的MS培养基中，进行诱芽和诱根培养，以MS BA2～4 IBA0.2～0.25和MS BA2～6 NAA0.2～0.3适合诱芽生长和继代培养；诱芽率77.7%～100%；芽增殖系数5.66～6.00和5.44～6.33,以MS IBA2～4 NAA0.2～0.4适合诱根生长；诱根率达306.6%～390.0%，诱芽生长最适温度25～30℃，诱根生长最适温度20～25℃；完整组培苗移栽成活率达92%。