植物乳杆菌LAI的酵母表面展示

为了研究亚油酸异构酶(LAI)的功能并构建基因工程菌株,克隆了植物乳杆菌1p15-2-1的lai基因并对其编码序列进行分析比对,随后将其融合酵母信号肽序列和0-凝集素锚定序列,构建酵母表面展示载体；使用电击法转化酿酒酵母K601,并在尿嘧啶缺失型培养基上筛选出转化子.氨基酸序列比对分析表明,LAI与肌球蛋白交叉反应抗原...     

Influence of green tea polyphenols on mitochondrial permeability transition pore and Ca^2＋ transport

The authors investigated the influence of green tea polyphenols(GPSs) on the liver mitochondria permeability transition pore(PTP) opening through mitochondria sweling and change of mitochcodrin membrane potetial .The data showed that GPSs had obvious protective effect on the Ca^2 -induced PTP opening in a dose-dependent manner detected by mitochondria swelling.The results were obtained by meating the change of mitochondria membrane potential through Rh 123.Further experiments were conducted to examine the detailed influence of GTPs on Ca^2 import and export of mitochondria.The results showed that GTPs had remarkably inhibitory effect on the Ca^2 -induced Ca^2 import in mitochondria.and they could accelerate Ca^2 -release from from mitochondria.Our data provide an altemate interpretation of the potent protective function of GPSs on cell against apoptosis.