Activation of Calf Intestinal Alkaline Phosphatase by Trifluoroethanol

IntroductionAlkaline phosphatase(EC3 .1 .3 .1 ) is a nonspecificphosphomonoesterase that is found in bothprocaryotes and eucaryotes as a dimer withidentical subunits[1] .It belongs to the group ofenzymes attached to the lipid bilayer of membranesby a glycosylphosphatidylinositol anchor[2 4] .Itis ametalloprotein that has two Zn2 +ions and oneMg2 +ion in each active site.Both its catalyticmechanism and structure have been thoroughlystudied and reviewed[5,6 ] .In mammals,alkalinephosphatase is…     

Enhancement of Aminoacylase Activity by Sodium Citrate

IntroductionEnzyme activation is a common phenomenon inbiological studies. Many experiments havesuggested that enzyme activation is caused by theconformational change of the enzyme activesites[14 ] . Other papers have showed that theenhancement of enzyme activity is associated withsecondary or tertiary structural changes[5] .This study analyses aminoacylase (N-acylamino acid amidohydrolase,EC3 .5 .1 .1 4) ,adimeric protein,consisting of two identicalsubunits each with an active site. The zin…