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A R Fersht J P Shi J Knill-Jones D M Lowe A J Wilkinson D M Blow P Brick P Carter M M Waye G Winter 《Nature》1985,314(6008):235-238
The role of complementary hydrogen bonding as a determinant of biological specificity has been examined by protein engineering of the tyrosyl-tRNA synthetase. Deletion of a side chain between enzyme and substrate to leave an unpaired, uncharged hydrogen-bond donor or acceptor weakens binding energy by only 0.5-1.5 kcal mol-1. But the presence of an unpaired and charged donor or acceptor weakens binding by a further approximately 3 kcal mol-1. 相似文献
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