全文获取类型
收费全文 | 8216篇 |
免费 | 163篇 |
国内免费 | 244篇 |
专业分类
系统科学 | 160篇 |
丛书文集 | 171篇 |
教育与普及 | 130篇 |
理论与方法论 | 16篇 |
现状及发展 | 1981篇 |
研究方法 | 216篇 |
综合类 | 5937篇 |
自然研究 | 12篇 |
出版年
2022年 | 64篇 |
2015年 | 78篇 |
2014年 | 115篇 |
2013年 | 113篇 |
2012年 | 173篇 |
2011年 | 226篇 |
2010年 | 163篇 |
2009年 | 260篇 |
2008年 | 242篇 |
2007年 | 253篇 |
2006年 | 215篇 |
2005年 | 249篇 |
2004年 | 252篇 |
2003年 | 221篇 |
2002年 | 254篇 |
2001年 | 390篇 |
2000年 | 306篇 |
1999年 | 387篇 |
1998年 | 242篇 |
1997年 | 201篇 |
1996年 | 164篇 |
1995年 | 183篇 |
1994年 | 153篇 |
1993年 | 127篇 |
1992年 | 221篇 |
1991年 | 199篇 |
1990年 | 203篇 |
1989年 | 174篇 |
1988年 | 159篇 |
1987年 | 143篇 |
1986年 | 86篇 |
1985年 | 112篇 |
1984年 | 82篇 |
1983年 | 62篇 |
1982年 | 65篇 |
1980年 | 75篇 |
1979年 | 146篇 |
1978年 | 134篇 |
1977年 | 107篇 |
1976年 | 79篇 |
1975年 | 73篇 |
1974年 | 98篇 |
1973年 | 103篇 |
1972年 | 89篇 |
1971年 | 111篇 |
1970年 | 100篇 |
1969年 | 96篇 |
1968年 | 95篇 |
1967年 | 87篇 |
1966年 | 86篇 |
排序方式: 共有8623条查询结果,搜索用时 0 毫秒
1.
Patricio Atanes Inmaculada Ruz-Maldonado Ross Hawkes Bo Liu Min Zhao Guo Cai Huang Israa Mohammed Al-Amily Albert Salehi Stefan Amisten Shanta J. Persaud 《Cellular and molecular life sciences : CMLS》2018,75(16):3039-3050
Introduction
Islets synthesise and secrete numerous peptides, some of which are known to be important regulators of islet function and glucose homeostasis. In this study, we quantified mRNAs encoding all peptide ligands of islet G protein-coupled receptors (GPCRs) in isolated human and mouse islets and carried out in vitro islet hormone secretion studies to provide functional confirmation for the species-specific role of peptide YY (PYY) in mouse islets.Materials and methods
GPCR peptide ligand mRNAs in human and mouse islets were quantified by quantitative real-time PCR relative to the reference genes ACTB, GAPDH, PPIA, TBP and TFRC. The pathways connecting GPCR peptide ligands with their receptors were identified by manual searches in the PubMed, IUPHAR and Ingenuity databases. Distribution of PYY protein in mouse and human islets was determined by immunohistochemistry. Insulin, glucagon and somatostatin secretion from islets was measured by radioimmunoassay.Results
We have quantified GPCR peptide ligand mRNA expression in human and mouse islets and created specific signalomes mapping the pathways by which islet peptide ligands regulate human and mouse GPCR signalling. We also identified species-specific islet expression of several GPCR ligands. In particular, PYY mRNA levels were ~ 40,000-fold higher in mouse than human islets, suggesting a more important role of locally secreted Pyy in mouse islets. This was confirmed by IHC and functional experiments measuring insulin, glucagon and somatostatin secretion.Discussion
The detailed human and mouse islet GPCR peptide ligand atlases will allow accurate translation of mouse islet functional studies for the identification of GPCR/peptide signalling pathways relevant for human physiology, which may lead to novel treatment modalities of diabetes and metabolic disease.2.
In this paper,the new development of three basic theories of Extenics,incluing basic-element theory,extension set theory and extension logic theory ,has been introduced.And the new theoretical frame of Extenics has also been set forth. 相似文献
3.
杨建荣 《科技情报开发与经济》2004,14(6):157-158
根据我国当前电力系统运行情况,对电网无功功率补偿的必要性及通用补偿方法及其装置进行了具体阐述。 相似文献
4.
RAOLan-lan CAIDong-han 《武汉大学学报:自然科学英文版》2004,9(4):404-406
We prove that the model with physical and human capital adjustment costs has optimal solution when the production function is increasing return and the structure of vetor fields of the model changes substantially when the prodution function from decreasing return turns to increasing return. And it is shown that the economy is improved when the coefficients of adjustment costs become small. 相似文献
5.
西文期刊回溯建库中几个重要项目的著录 总被引:4,自引:0,他引:4
在实践的基础上,从期刊的出版日期、出版周期、卷、期、年、月及其他标识、ISSN、增刊、出版者以及期刊的历史发展等方面对西文期刊机读目录的标准化著录问题进行了探讨。 相似文献
6.
王永红 《科技情报开发与经济》2004,14(6):139-141
通过1998年、2000年的试验研究,系统分析了顶凌覆盖技术在高寒区节水增温保温、增产增收的机理及其应用效果。提出该技术可以缓解低温干旱特别是春旱对农业生产的困扰,是一项可以抗旱保春播增产增收的实用技术,在高寒地区具有较高的推广价值。 相似文献
7.
8.
Y Y He T McNally I Manfield O Navratil I G Old S E Phillips I Saint-Girons P G Stockley 《Nature》1992,359(6394):431-433
The three-dimensional crystal structure of the Escherichia coli methionine repressor, MetJ, complexed with a DNA operator fragment is described in an accompanying article. The complex exhibits several novel features of DNA-protein interaction. DNA sequence recognition is achieved largely by hydrogen-bond contacts between the bases and amino-acid side chains located on a beta-ribbon, a mode of recognition previously hypothesized on the basis of modelling of idealized beta-strands and DNA, and mutagenesis of the Salmonella phage P22 repressors Arc and Mnt. The complex comprises a pair of MetJ repressor dimers which bind to adjacent met-box sites on the DNA, and contact each other by means of a pair of antiparallel alpha-helices. Here we assess the importance of these contacts, and also of contacts that would be made between the C-helices of the protein and DNA in a previous model of the complex, by studying mutations aimed at disrupting them. The role of the carboxy-terminal helix face in operator binding was unclear, but we demonstrate that recognition of operator sequences occurs through side chains in the beta-strand motif and that dimer-dimer interactions are required for effective repression. 相似文献
9.
小麦群体连续选择效应的综合分析 总被引:1,自引:1,他引:0
本试验对由信谷核不育基因构成的异效群体内4个性状进行连续两轮歧化选择,并将所得数据进行群体综合分析。结果表明:整个群体的平均表现和变异程度未发明显变化;但群体分布状况有一定改变,尤其是每穗粒数的分布数化较为明显;株高和千粒重的有效因子数估计基本符合小麦遗传实际表现,每穗粒数的有效有效因子数估计略低,说明此性状遗传背景较为简单。 相似文献
10.
金刚石薄膜是工业界继高温超导材料之后的又一研究热点,近年来的研究工作已经取得了重大进展。文章对国内外学者在金刚石薄膜的制备、特性、生长机理以及摩擦特性等方面的研究进行了综合论述,以推动国内外金刚石薄膜基础研究和应用的发展,并提出要重视金刚石薄膜在摩擦学领域中的应用研究,使之为人类社会带来巨大的经济效益。 相似文献