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排序方式: 共有98条查询结果,搜索用时 16 毫秒
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S Peña-Llopis S Vega-Rubín-de-Celis A Liao N Leng A Pavía-Jiménez S Wang T Yamasaki L Zhrebker S Sivanand P Spence L Kinch T Hambuch S Jain Y Lotan V Margulis AI Sagalowsky PB Summerour W Kabbani SW Wong N Grishin M Laurent XJ Xie CD Haudenschild MT Ross DR Bentley P Kapur J Brugarolas 《Nature genetics》2012,44(9):1072
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Two non-standard techniques – the Rasch Model and Nonlinear Principal Components Analysis – originally proposed in other fields
are presented and discussed to measure the opinion of European citizens about utilities. The Eurobarometer survey data are
thus considered. The potential of both methods and their complementary use are highlighted; in particular the methods allow
the questionnaire to be calibrated, the role of different services and aspects of service to be established, and the consumer
satisfaction to be assessed and compared among European countries, different years and services. 相似文献
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Although molecular data have revealed the vast scope of microbial diversity, two fundamental questions remain unanswered even for well-defined natural microbial communities: how many bacterial types co-exist, and are such types naturally organized into phylogenetically discrete units of potential ecological significance? It has been argued that without such information, the environmental function, population biology and biogeography of microorganisms cannot be rigorously explored. Here we address these questions by comprehensive sampling of two large 16S ribosomal RNA clone libraries from a coastal bacterioplankton community. We show that compensation for artefacts generated by common library construction techniques reveals fine-scale patterns of community composition. At least 516 ribotypes (unique rRNA sequences) were detected in the sample and, by statistical extrapolation, at least 1,633 co-existing ribotypes in the sampled population. More than 50% of the ribotypes fall into discrete clusters containing less than 1% sequence divergence. This pattern cannot be accounted for by interoperon variation, indicating a large predominance of closely related taxa in this community. We propose that such microdiverse clusters arise by selective sweeps and persist because competitive mechanisms are too weak to purge diversity from within them. 相似文献
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Summary An extracellular protease has been purified from cultures ofPseudomonas fluorescens. It is a metalloenzyme with a molecular weight of 37,000±3,700, able to digest casein, hemoglobin and gelatine.This work was supported by grants from the Consejo Nacional de Investig ciones Científicas y Técnicas de la República Argentina, and the Consejo de Investigaciones de la Universidad Nacional de Rosario. JJC is a member of the Carrera del Investigador Científico, and SMJ the recipient, of a schollarship, of the former institution. 相似文献
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Silvia Bassani Alessandra Folci Jonathan Zapata Maria Passafaro 《Cellular and molecular life sciences : CMLS》2013,70(23):4411-4430
Glutamate ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (AMPARs) mediate most fast excitatory synaptic transmission in the central nervous system. The content and composition of AMPARs in postsynaptic membranes (which determine synaptic strength) are dependent on the regulated trafficking of AMPAR subunits in and out of the membranes. AMPAR trafficking is a key mechanism that drives nascent synapse development, and is the main determinant of both Hebbian and homeostatic plasticity in mature synapses. Hebbian plasticity seems to be the biological substrate of at least some forms of learning and memory; while homeostatic plasticity (also known as synaptic scaling) keeps neuronal circuits stable by maintaining changes within a physiological range. In this review, we examine recent findings that provide further understanding of the role of AMPAR trafficking in synapse maturation, Hebbian plasticity, and homeostatic plasticity. 相似文献
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Rong Grace Zhai Menico Rizzi Silvia Garavaglia 《Cellular and molecular life sciences : CMLS》2009,66(17):2805-2818
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase (NMNAT) has long been known as the master enzyme in NAD biosynthesis
in living organisms. A burst of investigations on NMNAT, going beyond enzymology, have paralleled increasing discoveries of
key roles played by NAD homeostasis in a number or patho-physiological conditions. The availability of in-depth kinetics and
structural enzymology analyses carried out on NMNATs from different organisms offer a powerful tool for uncovering fascinating
evolutionary relationships. On the other hand, additional functions featuring NMNAT have emerged from investigations aimed
at unraveling the molecular mechanisms responsible for complex biological phenomena such as neurodegeneration. NMNAT appears
to be a multifunctional protein that sits both at the core of central metabolism and at a crossroads of multiple cellular
processes. The resultant wealth of biochemical data has built a robust framework upon which design of NMNAT activators, inhibitors
or enzyme variants of potential medical interest can be based. 相似文献