耐水脱硫石膏、粉煤灰胶结材的研究

本文较深入地研究了脱硫石膏粉煤灰化学成分、杂质、颗粒特征和激发剂等对脱硫石膏、粉煤灰胶结材性能的影响 研究表明 ,激发剂是影响脱硫石膏、粉煤灰胶结材性能的主要因素 这为脱硫石膏、粉煤灰资源化利用打下良好理论基础 经实际应用表明 ,研究结果与实际情况能很好相符     

Mammalian toll-like receptors: from endogenous ligands to tissue regeneration

Following injury a complex but well-orchestrated cellular response stimulating wound healing and tissue regeneration is induced. The balance of different cytokines, growth factors and cells is important in regulating tissue reorganisation. The immune system is critically involved in this process. Toll-like receptors (TLRs) are essential to the innate immune system, recognising microbial pathogens. The recent identification of endogenous ligands of TLRs suggests that they function not only to induce defensive antimicrobial immune responses but also as a sensitive detection system to initiate tissue regeneration after injury. Here we present an overview of TLRs and their endogenous ligands, and also review the roles of TLRs in inducing tissue regeneration after injury and in maintaining homeostasis. The identification of endogenous TLR ligands and their involvement in inducing tissue regeneration will provide new options to improve tissue reorganization after injury. Received 26 April 2006; received after revision 16 June 2006; accepted 24 August 2006     

Apoptosis induced by titanium dioxide nanoparticles in cultured murine microglia N9 cells

Owing to the rapidly increasing output of nano-scale titanium dioxide （TiO2） particles, their potential risk for central nerve system （CNS） has elicited much concern recently. Microglia is the resident macrophage in CNS and essential for the homeostasis of the CNS microenvironment. They are supposed to response to nanoparticles depositing in the brain tissues. Therefore, we investigated the cytotoxic effects of TiO2 NPs on microglia N9 cells in vitro. Results of propidium iodide/fluorescein diacetate （PI/FDA） double staining and MTT test clearly showed that TiO2 NPs more efficiently affected the viability of microglia N9 cells. Further Hoechst 33258 staining and flow cytometric analysis proved that nano-scale but not normal scale TiO2 induced apoptosis in vitro, These data suggest that TiO2 NPs can elicit apoptosis of N9 cells in vitro and thus present a potential risk for CNS.     

Valproic acid attenuates inflammation in experimental autoimmune neuritis

Valproic acid (VPA) is a short-chain branched fatty with anti-inflammatory, neuro-protective and axon-remodeling effects. We investigated the effects of VPA in rats in which experimental autoimmune neuritis (EAN) had been induced (EAN rats). VPA (300 mg/kg, intraperitoneally) administration to EAN rats once daily immediately following immunization significantly suppressed mRNA levels of interferon-γ, tumor necrosis factor-α, interleukin (IL)-1β, IL-4, IL-6 and IL-17 in the lymph nodes of EAN rats. In peripheral blood and sciatic nerves of EAN rats, Foxp3⁺ cells were increased but IL-17⁺ cells were decreased during VPA treatment. Furthermore, suppressive and therapeutic treatment with VPA greatly attenuated both accumulation of macrophages, T cells and B cells, and demyelination in sciatic nerves, and greatly reduced the severity and duration of EAN. In summary, our data demonstrated that VPA could effectively suppress inflammation in EAN, suggesting that VPA could be a potent candidate for treatment of autoimmune neuropathies.     

The innate immunity of the central nervous system in chronic pain: The role of Toll-like receptors

Toll-like receptors (TLRs) are a family of pattern recognition receptors that mediate innate immune responses to stimuli from pathogens or endogenous signals. Under various pathological conditions, the central nervous system (CNS) mounts a well-organized innate immune response, in which glial cells, in particular microglia, are activated. Further, the innate immune system has emerged as a promising target for therapeutic control of development and persistence of chronic pain. Especially, microglial cells respond to peripheral and central infection, injury, and other stressor signals arriving at the CNS and initiate a CNS immune activation that might contribute to chronic pain facilitation. In the orchestration of this limited immune reaction, TLRs on microglia appear to be most relevant in triggering and tailoring microglial activation, which might be a driving force of chronic pain. New therapeutic approaches targeting the CNS innate immune system may achieve the essential pharmacological control of chronic pain. Received 21 November 2006; received after revision 8 January 2007; accepted 7 February 2007     

正弦稳态扫频信号的产生与输出

介绍用计算机软件产生正弦稳态扫频信号的编程方法。通过对Ｄ／Ａ变换与Ａ／Ｄ采集的同步输出可以获得不同频率量程和设定电压的扫频输出     

一类高阶非线性系统的零解稳定性

由Барбащин公式得到的四阶常系数线性系统的Ляпунов函数出发，通过类比法构造了一类四阶非线性系统的Ляпунов函数，并由此得到了这些系统零解的全局渐近稳定性的充分条件     

In vivo and in vitro differences between leukocytic uptake of oligodeoxynucleotides

Development and application of therapeutic oligonucleotides rely on proper analysis of binding and uptake. We have used several model oligodeoxynucleotides (ODNs) to analyze binding/uptake by rat and human leukocytes. Here we describe: (1) differences between in vivo and in vitro uptake of ODNs to rat leukocytes, (2) differences after injection of lipopolysaccharide (LPS), (3) large in vitro differences between primary mononuclear cells in PBS, plasma and blood, and (4) differences of ODN uptake between rat and human leukocytes. Our data show that ODN uptake by primary blood cells was different in PBS, plasma and blood. In addition, LPS treatment increased ODN uptake by leukocytes in blood, indicating that pathological conditions may influence ODN uptake. Furthermore, ODN uptake in rat and human blood is also different, suggesting that preclinical ODN uptake data from rat blood cannot easily be extrapolated to the human condition. Received 17 December 2007; received after revision 16 January 2008; accepted 5 February 2008