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The annexins: spatial and temporal coordination of signaling events during cellular stress 总被引:2,自引:0,他引:2
Katia Monastyrskaya Eduard B. Babiychuk Annette Draeger 《Cellular and molecular life sciences : CMLS》2009,66(16):2623-2642
Annexins are a family of structurally related, Ca2+-sensitive proteins that bind to negatively charged phospholipids and establish specific interactions with other lipids and
lipid microdomains. They are present in all eukaryotic cells and share a common folding motif, the “annexin core”, which incorporates
Ca2+- and membrane-binding sites. Annexins participate in a variety of intracellular processes, ranging from the regulation of
membrane dynamics to cell migration, proliferation, and apoptosis. Here we focus on the role of annexins in cellular signaling
during stress. A chronic stress response triggers the activation of different intracellular pathways, resulting in profound
changes in Ca2+ and pH homeostasis and the production of lipid second messengers. We review the latest data on how these changes are sensed
by the annexins, which have the ability to simultaneously interact with specific lipid and protein moieties at the plasma
membrane, contributing to stress adaptation via regulation of various signaling pathways. 相似文献
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Monastyrskaya K Babiychuk EB Schittny JC Rescher U Gerke V Mannherz HG Draeger A 《Cellular and molecular life sciences : CMLS》2003,60(12):2702-2709
Relaying a signal across the plasma membrane requires functional connections between the partner molecules. Membrane microdomains or lipid rafts provide an environment in which such specific interactions can take place. The integrity of these sites is often taken for granted when signalling pathways are investigated in cell culture. However, it is well known that smooth muscle and endothelial cells undergo cytoskeletal rearrangements during monolayer culturing. Likewise affected – and with potentially important consequences for signalling events – is the organization of the plasma membrane. The expression levels of three raft markers were massively upregulated, and raft-associated 5-nucleotidase activity increased in conventional monolayer cultures as compared with a spheroidal coculture model, shown to promote the differentiation of endothelial cells. Our data point to a shift of raft components in monolayer cultures and demonstrate potential advantages of the spheroid coculture system for investigation of raft-mediated signalling events in endothelial cells.Received 4 August 2003; received after revision 18 September 2003; accepted 25 September 2003 相似文献
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