棉纤维相关cDNA的克隆及其在4个纤维突变体中的结构变异

用ＲＴ－ＰＣＲ方法从陆地棉遗传标准系“ＴＭ－１”的纤维细胞ＲＮＡ中克隆了一种纤维细胞优势表达的ｍＲＮＡ（ｃＤＮＡ，记为ＴＭ－Ｅ６基因），该基因不含内含子，可读框长为７７１ｂｐ，可编码２４６个氨基酸的多肽。从４个纤维突变体中分别克隆同源性基因，并与野生型“ＴＭ－１”的ＴＭ－Ｅ６基因进行了同源性分析，发现纤维突变体不但在基因结构而且在密码组成上有明显变异。在距转录起始位点的第２７９碱基对处有ＧＧＣＴＣ     

A suppressed gene in integument cells of a fiberless seed mutant in upland cotton

A fiberless seed mutant(fl) was identified in a commercial cotton(Gossypium hirsutum L.) variety Xu-Zhou 142(FL).THis phenotype is associated with lack of fiber cell initiation in the outer integument of the ovule,as was characterized by analysis of genes related to fiber differentiation and development.Two genes,fl-E6 and FL-E6,were cloned from fl-integument cells and FL-fiber or integument cells,respectively.Compared with FL-E6,fl-E6 showed a dramatic change in nucleotide sequence:(1) FL-E6 contained a tandem repetitive sequence in which GGCTCA( Gly-Ser) is repeated five times between the 82nd and the 93rd codon from the first ATG codon,while in fl-E6 the same sequence is repeated four times;(2) The fl-E6 gene encodes a polypeptide of 241 amino acids but lacks two codons between the 90th and 93rd codon and three between the 171st and 174th relative to FL-E6;(3) There are also 12 nucleotide substitutions which would result in 7 amino acid differences between fl-E6 and FL-E6.Analysis of RT-PCR and Northern Blot showed that expression of the fl-E6 gene is suppressed in the fl-integument cells.but highly expressed in FL-fiber cells.The difference between fl-E6 and FL-E6 may be associated with lower expression of fl-E6 in the fli-inbackbones of two arabinogalactan-proteins(AGPs),one from the filtrate of suspension-cultured cells of Pyrus communis (AGPPc2) and the other from Nicotiana alata(AGPNa2),Although the function of the FL-E6 protein in differentation and development of cotton fiber cells is not known,the data indicate that the mutation of fl-E6 gene from FL-E6 gene may inhibit the fiber cell initiation from epidermal cells of the outer integument of the ovule.