Mechanisms regulating immune surveillance of cellular stress in cancer

The purpose of this review is to explore immune-mediated mechanisms of stress surveillance in cancer, with particular emphasis on the idea that all cancers have classical hallmarks (Hanahan and Weinberg in Cell 100:57–70, 67; Cell 144:646–674, 68) that could be interrelated. We postulate that hallmarks of cancer associated with cellular stress pathways (Luo et al. in Cell 136:823–837, 101) including oxidative stress, proteotoxic stress, mitotic stress, DNA damage, and metabolic stress could define and modulate the inflammatory component of cancer. As such, the overarching goal of this review is to define the types of cellular stress that cancer cells undergo, and then to explore mechanisms by which immune cells recognize, respond to, and are affected by each stress response.     

Hydrogen-poor superluminous stellar explosions

Supernovae are stellar explosions driven by gravitational or thermonuclear energy that is observed as electromagnetic radiation emitted over weeks or more. In all known supernovae, this radiation comes from internal energy deposited in the outflowing ejecta by one or more of the following processes: radioactive decay of freshly synthesized elements (typically (56)Ni), the explosion shock in the envelope of a supergiant star, and interaction between the debris and slowly moving, hydrogen-rich circumstellar material. Here we report observations of a class of luminous supernovae whose properties cannot be explained by any of these processes. The class includes four new supernovae that we have discovered and two previously unexplained events (SN 2005ap and SCP 06F6) that we can now identify as members of the same class. These supernovae are all about ten times brighter than most type Ia supernova, do not show any trace of hydrogen, emit significant ultraviolet flux for extended periods of time and have late-time decay rates that are inconsistent with radioactivity. Our data require that the observed radiation be emitted by hydrogen-free material distributed over a large radius (～10(15) centimetres) and expanding at high speeds (>10(4) kilometres per second). These long-lived, ultraviolet-luminous events can be observed out to redshifts z?>?4.     

Type VI secretion delivers bacteriolytic effectors to target cells

Peptidoglycan is the major structural constituent of the bacterial cell wall, forming a meshwork outside the cytoplasmic membrane that maintains cell shape and prevents lysis. In Gram-negative bacteria, peptidoglycan is located in the periplasm, where it is protected from exogenous lytic enzymes by the outer membrane. Here we show that the type VI secretion system of Pseudomonas aeruginosa breaches this barrier to deliver two effector proteins, Tse1 and Tse3, to the periplasm of recipient cells. In this compartment, the effectors hydrolyse peptidoglycan, thereby providing a fitness advantage for P. aeruginosa cells in competition with other bacteria. To protect itself from lysis by Tse1 and Tse3, P. aeruginosa uses specific periplasmically localized immunity proteins. The requirement for these immunity proteins depends on intercellular self-intoxication through an active type VI secretion system, indicating a mechanism for export whereby effectors do not access donor cell periplasm in transit.     

数字测温电桥参数的一种计算方法

用INTESIL 7106，7107等芯片作为A／D转换器，以铂热电阻、铜热电阻作测温器件组成的数字电压表，具有成本低、性能稳定、维修方便等优点，便为校正读数的非线性，常需采用专门的校正电路，实则在量程不太宽时，适当选择电桥参数，使读数误差在整个量程内均匀分布，既使不采用校正电路也可满足一般要求，本文给出这种计算测温电桥电路的工程方法。     

Complex Network Analysis of the Robustness of the Hanoi,Vietnam Bus Network

Many complex networks exist to facilitate the transport of material or information. In this capacity, the authors are often concerned with the continued flow of material or information when a fraction of the links in the complex network is disrupted. In other words, the authors are interested in the robustness of the complex network. In this paper, the authors survey measures of robustness like the average path length, the average clustering coefficient, the global efficiency, the size of largest cluster and use these to analyze the robustness of the bus network in Hanoi, Vietnam. The authors find that the bus network is robust against random failure but sensitive to targeted attack, in agreement with its scale-free character. By examining sharp drops in the average path length within the largest cluster of the Hanoi bus network under successive targeted attack, the authors identify five nodes whose loss lead to the fragmentation of the network into five or six disconnected clusters. These isolated clusters represent geographically the Central, Western, Southern, and Northwestern districts of Hanoi. Special considerations must therefore be given to these five nodes when planners wish to expand the bus network, or make it more robust.     

Stochastic systems arising from Markov modulated empirical measures

This work is devoted to stochastic systems arising from empirical measures of random sequences (termed primary sequences) that are modulated by another Markov chain. The Markov chain is used to model random discrete events that are not represented in the primary sequences. One novel feature is that in lieu of the usual scaling in empirical measure sequences, the authors consider scaling in both space and time, which leads to new limit results. Under broad conditions, it is shown that a scaled sequence of the empirical measure converges weakly to a number of Brownian bridges modulated by a continuous-time Markov chain. Ramifications and special cases are also considered.     

Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library.

A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.     

水声通信信道中的OPNET建模与仿真

针对水声通信网络信道的实现开销大、复杂性高的特点,提出了一种能够仿真分析水声通信网络的有效方法.该方法分别使用OPNET中的Propagation-Delay-Stage,Receiver-Power-Stage和Background-Noise-Stage三个工具来仿真水声信道中的传播延迟、发射机功率和水声噪声(水声噪声包括紊流、船运、风波和热噪声).其中,Propagation-Delay-Stage采用MacKenzie速度模型;Receiver-Power-Stage中有Thorp,Schulkin&Marsh和Francois&Garrison 3种传播损失模型.在该方法中,首先仿真比较了取不同传播损失模型时的水声信道,然后根据仿真结果选择一个合适的水声信道,并采用MACAW协议来仿真水声通信网络.最后,通过仿真实验对整个水声通信网络的吞吐量、误码率、丢包率进行了分析.实验结果表明,采用该方法能够有效地仿真水声通信网络.