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目的: 为了解析芽殖酵母 Num1 蛋白在纺锤体定位和线粒体中的调节机制.方法: 利用大肠杆菌原核表达
并纯化了在 Num1 功能中起核心作用的补丁组装(PA) 结构域的重组蛋白, 通过蛋白体外结合实验(Pull-down) 分
离了酵母细胞中与 PA 结构域相结合的蛋白复合物,并对其进行了质谱分析.结果: 鉴定了一系列新的 Num1 互作蛋
白,包括核糖体蛋白,参与蛋白折叠、分配及转运的内质网和高尔基复合体蛋白, 参与基因转录和翻译的核酸酶和
蛋白酶,及其他功能的蛋白.结论: Num1 的互作蛋白的鉴定为进一步研究 Num1 的作用机制奠定了基础. 相似文献
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A. Gerson-Gurwitz N. Movshovich R. Avunie V. Fridman K. Moyal B. Katz M. A. Hoyt L. Gheber 《Cellular and molecular life sciences : CMLS》2009,66(2):301-313
S. cerevisiae anaphase spindle elongation is accomplished by the overlapping function of dynein and the kinesin-5 motor proteins, Cin8
and Kip1. Cin8 and dynein are synthetically lethal, yet the arrest phenotypes of cells eliminated for their function had not
been identified. We found that at a non-permissive temperature, dyn1Δ cells that carry a temperature-sensitive cin8 – 3 mutation arrest at mid-anaphase with a unique phenotype, which we named TAN (two microtubule asters in one nucleus). These
cells enter anaphase, but fail to proceed through the slow phase of anaphase B. At a permissive temperature, dyn1Δ, cin8 – 3 or dyn1Δcin8 – 3 cells exhibit perturbed spindle midzone morphologies, with dyn1Δcin8 – 3 anaphase spindles also being profoundly bent and nonrigid. Sorbitol, which has been suggested to stabilize microtubules,
corrects these defects and suppresses the TAN phenotype. We conclude that dynein and Cin8 cooperate in anaphase midzone organization
and influence microtubule dynamics, thus enabling progression through the slow phase of anaphase B.
Received 10 August 2008; received after revision 22 October 2008; accepted 27 October 2008 相似文献
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The kinetochore is structurally composed of four layers,We know that three microtubule-based motor protcins such as CENP-E,dynein,and MCAK are located at the outmost region of the kinctochore,Experimentation of these motot functions betters our understanding of mitotic regulation,and chromaosme movements in particular,With real-time studies of chromosome movements in live cells,we hope to illustrate the molecular mechanisms under lying mitotic regulation. 相似文献
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Amos LA 《Cellular and molecular life sciences : CMLS》2008,65(4):509-515
Models commonly used to explain the mechanism of myosin motors typically include a power stroke that is attributed to a conformational
change in the motor domain and amplified by a long lever arm that connects the motor domain to the cargo. Similar models have
proved less enlightening in the case of microtubule motors, for which it may be more helpful to consider models involving
thermally driven mechanisms.
Received 9 November 2007; received after revision 7 December 2007; accepted 11 December 2007 相似文献
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